| Freeze Substitution Applications |
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Applications with the Leica EM AFS2 + EM FSP
Automatic Freeze Substitution System |
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Freeze Substitution of specimens in methanol, acetone or any other FS media at low temperature is THE follow-on precedure to High Pressure Freezing (Leica EM PACT2) and other cryo fixation methods.
Progressive Lowering of Temperature (PLT) allows to substitute and resin infiltrate chemically fixed specimens.
Finally the sample is polymerised under UV light in the Leica EM AFS2 and can be cut and immuno labelled. |
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| Cells of Saccharomyces cerevisiae were grown to stationary phase. Then they were cryofixed and freeze-substituted with Leica EM A ... |
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| Piece of cartilage from the hip of a mouse were cryofixed with the Leica EM PACT and freeze-substituted in acetone containing 0,5 ... |
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| Liver HEPG2 cells were grown in suspension on microcarrier. Then they were cryofixed with the Leica EM PACT and freeze-substituted ... |
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| Rat, kidneyCourtesy of L. Edelmann,University Homburg, Germany ... |
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