Saccharomyces cerevisiae

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Cells of Saccharomyces cerevisiae were grown to stationary phase. Then they were cryofixed and freeze-substituted with Leica EM AFS2 in acetone containing 0.5 % glutaraldehyde and 0.2 % uranyl acetate and low-temperature embedded in Lowicryl HM20. Bar represents: 10 µm Courtesy of: E.G. van Donselaar, B.M. Humbel Electron Microscopy & Structure Analysis, Department of Molecular Cell Biology, Utrecht University, The Netherlands J.W.Slot Department of Cell Biology, University Medical Center Utrecht, The Netherlands