| Immunogold Labelling |
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Applications with the Leica EM IGL
The first Automated Immunolabelling System |
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The Leica EM IGL meets the demands of today's immunolabelling laboratory.By preparing all your reagents together, you can set up a run within a few minutes and free your time for more productive activities.Reagent droplets are placed in humidified carries and moved automatically to grids according to the programme in use. |
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| Hints and TipsHow to convert a manual immuno-incubation protocol for ultrathin sections into a protocol suitable for use w ... |
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| Incubated with cholesterol marker coupled to biotin. Bar = 200 nm P = Plasma membraneE = Endosomes/lysosomeBar = 200 n ... |
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| Immunogold labelling of catalase on liver cryo sectionsBar = 1 µmCourtesy of: JEOL, International Training Centre Japan an ... |
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| Cryo sectioning of HepG2-hepatome. Labelled for Cathepsin DMonoclonal mouse anti-human dilutedin PBS/BSA 1 %Visual ... |
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| Immunogold/silver labeling of alphaamylase in Lowicryl HM20 embedded pancreas tissue using Aurion ultrasmall gold conjugate and R- ... |
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| Modified Vaccinia Virus Ankara (MVA The B5R envelope protein is labelled with a rabbit anti-B5R antibody and detected with a g ... |
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| Human Dendritic cellMHC II compartment labelled with rabbit anti-alpha chain.Detected wth PAG coupled to 10 nm goldBar ... |
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| Rat Liver, peroxisomes labelled with rabbit anti-catalase antibodies.Detected with goat anti-rabbit ultra small gold particles ... |
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| Rat Liver embedded in LR white resin.Immuno labelling of Catalase. Detected with Goat anti-rabbitCourtesy of Bruno ... |
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| Immuno-incubation performed with Rabbit and rat amylase serum.The antibodies were visualized with protein A-Gold 10 nmS = secr ... |
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| The gp64 is important for adhesion to the target cells and for membrane fusion with the infected cell.The shown gp64 staining serv ... |
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