Ultrathin CNS slices: Enhanced visualization of neuron structure for electrophysiology and imaging

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Leica VT1000 S   Read the entire application report

Live/dead cell staining revealed that many cells remained viable for imaging and electrophysiological experimentation. In all CNS regions examined, neurons were easily identified for successful patch recordings. While not yet tested, use of an inverted microscope should permit high numerical aperture oil-immersion objectives to be used for simultaneous imaging experiments and the reduced slice thickness would hasten drug equilibration and washout times. Supported by the Canadian Neuroscience Network. Image: Immunostaining of CAA1 glial cells with GFAP (scale bar = 50 µm) (for slice culture procedures, see Parsley, C.P. et al. Society for Neurosci. Abstr. 230.5, 1996)