Near-monolayer sectioning of live CNS tissue
Applications

Immunostaining of CAA1 glial cells with GFAP (scale bar = 50 µm) (for slice culture procedures, see Parsley, C.P. et al. Society for Neurosci. Abstr. 230.5, 1996)
  Applications Report as pdf download (0,30 MB)
The in-situ study of sections of living tissue maintained in-vitro is a powerful method to elucidate many aspects of cellular and network function in the CNS, both in acute slice and long-term culture (organotypic) preparations. Recently, there has been an increased demand for technologies that enhance optical resolution at the synaptic/cellular level in order to better identify particular cell types or examine the properties of distinct spatial regions within individual cells.

Applications brief from Shawn Hochman et al (Univ. of Manitoba, Canada) and Claudia Dorenkamp (Leica Microsystems Nussloch GmbH)