Principle & use of the Leica SP1600 saw microtome
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  Leica SM2500 E sledge microtome
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  Leica SP1600 saw microtome
With the sectioning technique serial sections can be produced, as opposed to the sawing technique in which the user has to calculate the loss of material caused by the thickness of the diamond coated saw blade. The speed of the rotating blade is approximately 600 rpm. The built-in water cooling device prevents overheating of the object and removes saw dust from the cutting edge and thus prolongs the lift time of the saw blade. The section thickness is set manually with a knurled screw on the object arm.

Specimen Preparation:
The object arm can be mounted into the object holder. The height of the object has to be adjusted until the surface of the object is slightly above the upper edge of the saw blade. The surface of the block has to be trimmed. Trimming means, that a plane surface has to be cut prior to produce slices of a defined thickness.

Drawing: Sawing technique