| Receptor Micro-Autoradiography was developed for the tissue localization of drugs. Since drugs are generally small molecular weight compounds acting through weak binding to specific receptors with limited capacity, the method is designed to provide high resolution and high sensitivity, and at the same time to preserve microscopic tissue structure with the compound retained at its original site in vivo. This method has been tested before its introduction with compounds known localized and applied for several decades to the study of drugs, demonstrating its high diagnostic and predictive value. Receptor micro-autoradiography is based on the use of radiolabeled (predominantly 3H or 125I) compounds with high specific activity in conjunction with nuclear emulsion-coated slides and thin frozen sections cut in a cryostat.
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