Confocal Spectrophotometry
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Emission spectre of fluorochromes are scanned one step at a time over a defined spectrum (Lambdascan). In order to achieve a more efficient detection of the spectre of combined fluorochrome stainings than with the application of simultaneous scanning, sequential scanning with optimized excitation and detection for each fluorochrome is used.
U. Tauer, O. Hils
Leica Microsystems Heidelberg GmbH, Deutschland
Scientific and Technical Information
Special edition “Confocal microscopy”
CDR 4, pp. 15-27
October 2000
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