Multi-wavelength epi-illumination in fluorescence microscopy

Download Area 1 | 2 | 3

During its course of nearly 40 years of application and development this technique became one of the basic tools for routine and research work in biology, medicine, science and industry. The progress in incident light fluorescence microscopy was particularly determined by the research work of Ploem and his function as trend setter and also by the forward strategy of Leitz resp. Leica Wetzlar in developing the optical instruments required.
This course of development is described chronolo-gically in the present contrbution. A synoptical table of fluorescence microscopy techniques gives relevant information about microscopes, objectives, fluorochromes, light sources and filter systems concerning the fields of application and the methods referring to this.

J. S. Ploem, F. Walter
Leiden University, Netherlands
Scientific and Technical Information
Edition CDR 5, p. 1-16
December 2001