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What are the key factors necessary for producing high quality frozen sections?
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- A good clean sharp knife/blade.
- A good knife/blade clamping system.
- Correct knife angle.
- Properly adjusted anti-roll plate.
- Damage-free anti-roll plate.
- Good working microtome.
- Optimum cutting temperature for tissue.
- Tissue and matrix fresh and not dehydrated.
- Optimal embedded samples.
- Well-trimmed sample.
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