Leica MP FLIM2

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Fluorescence lifetime measurement tool using multiphoton excitation

Functional principle of MP FLIM process

Overview

Fluorescence Lifetime Microscopy with Multiphoton Excitation
Analytical Extension of Leica TCS SP2 AOBS Confocal System

With FLIM a variety of rather local parameters within cells can be measured, such as ion concentrations, intracellular signal transduction, FRET, and membrane potential. It is based on time-correlated single photon counting technology. In contrast to intensity imagining FLIM is insensitive to fluctuations in fluorochrome concentration and excitation light intensity.

The MP FLIM2 extension uses a multiphoton laser as excitation source and time reference providing all the advantages of multiphoton excitation for FLIM analysis, like deep tissue penetration, less autofluorescence, and less photobleaching outside the focus.

Key Features

  • Multiphoton laser as excitation source
  • Multiphoton advantages: deep tissue penetration, low autofluorescence, improves signal to noise ratio, less photobleaching outside the focus
  • Usage of a wide range of fluorochromes and fluorescent proteins
  • Sophisticated design minimizes effect of incident light thus enhancing signal to noise ratio
  • Full Spectral Confocal with four fluorescence detector channels
  • for regular intensity imaging
  • Retrofit of Leica TCS SP2 AOBS systems
  • Data analysis on independent workstation keeps the Confocal free
  • Fluorescence decay data for each single pixel
  • Single or multiple exponential decay analysis