Specimen orientation, Leica RM2255
      
How can it be avoided that thick/thin sections are produced, the section thickness varies from one section to another or even every second section is skipped?
FAQ Report
  1. Insufficient clamping of the blade, the knife holder, the specimen and/or the orientation might cause this problem: Please check if all levers are locked and screws are tightened.

    Cassette related problems?

    Underfilling of the cassette body, overfilling (excess of paraffin on the outside of the cassette has to be removed prior to clamping. Wrong clamping system chosen: standard clamp instead of cassette clamp compresses weak cassettes.

  2. Locking levers are not well lubricated or cleaned, causing them not to be completely fastened or stuck in a non-locked position: Remove all locking levers, clean off all paraffin and debris, apply a sparing amount of the provided Leica lubricant. Use only manufacturer recommended lubricants.
  3. You might use a blunt cutting edge: Please use a new blade or adjust the knife holder laterally.
  4. The pressure plate is damaged or not correctly adjusted (applies only to the knife holder E for the RM2100 series): Insert a new pressure plate or use a new knife holder. Re-adjust the pressure plate.
  5. The specimen block is too large or too hard: If possible, trim the specimen. Soften the block by soaking it in iced water. Try knife with a different profile (d-profile instead of c-profile).
  6. There might be excess of oil on the blade: Remove the oil with soft paper towel or Q-tip.