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Leica Microsystems FluoScout™ – Get the Most out of Your Fluorescence Microscope

To achieve excellent imaging results in fluorescence microscopy, the light source, fluorophores and filter cubes have to match each other perfectly. FluoScout™ enables you to choose the right filter cube and filter set for maximizing the excitation and emission efficiency of your fluorescent dyes or fluorescent proteins.

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Leica Microsystems FluoScout™ – the online tool for determining the optimal fluorescence filter cube set

Fluorescence microscopy

Nowadays, fluorescence  is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. Fluorescence microscopy even allows users to determine the distribution of a single molecule species, its amount and its localization inside a cell. Colocalization and interaction studies can be performed, and ion concentrations as well as intra- and intercellular processes like endocytosis and exocytosis can be observed. With the help of super-resolution fluorescence microscopy, it is even possible to image sub-resolution structures. Read more about fluorescence microscopy, quantitative fluorescence and super-resolution on Leica Science Lab.

FluoScout™ smart functions

  • Visible light spectrum shown for permanent orientation
  • Zoom function for a detailed view of spectra
  • Switch on and off relevant fluorescence spectra to get an overview of the feature you want to see
  • Measure transmission and emission by using a slider
  • Quick help tool showing the functions
  • Link creator for e-mailing or bookmarking the selected spectra
  • Contact button to directly get in touch with our support team
  • Tooltip explaining the functions by mouse-over

Leica Microsystems FluoScout™ – the online tool for determining the optimal fluorescence filter cube set

  • Choose light source, fluorophores and filter cube separately.
  • Get a recommendation of filter cubes based on the choice of fluorophores.
  • Collect spectra by one click in the quotation cart and get offers for your filter sets.

Fluorescence Light Microscopes

By today fluorescence microscopes used in research applications are based on a set of optical filters: an excitation filter, a dichroic beam splitter and an emission filter. The filters are often plugged in together in a filter cube (compound microscopes) or in a flat holder (mainly stereo microscopes). Whereas the excitation filter selects the wavelengths to excite a particular dye within the specimen, the emission filter serves as a kind of quality control by letting only the wavelengths of interest emitted by the fluorophore pass through. The dichroic mirror's purpose is to reflect light in the excitation band and transmit light in the emission band,  enabling the classic epifluorescence incident light illumination.

Fluorescence Stereo Microscopes

Leica Microsystems’ fluorescence stereo microscopes use the TripleBeam technology, a separate (third) beam path for fluorescence illumination of the sample without a dichroic mirror. Therefore one excitation filter for the illumination beam path and two emission filters, one for each observation beam path, are needed.

Read more about the TripleBeam principle on Leica Science Lab