Warning! You won't be able to use the quotation basket until you enable cookies in your Web browser.
Warning! Your Web browser is no longer supported. Please upgrade to a modern browser.

Light Sources

Optimal Fluorescence Excitation in the Specimen

Adequate fluorescence intensity can only be obtained if the light source is strong in the near UV

Ultra-high pressure mercury lamps and high pressure xenon lamps, two types of gas discharge lamp, are particularly suitable.

  • Mercury lamps have a characteristic line spectrum with high intensity Hg lines.
  • Xenon lamps emit a continuous spectrum in the visible region with a constant average intensity.

Ultra-high pressure mercury lamps are the most popular light sources for general fluorescence work due to their universal application potential, their strong ultra-violet, violet and green and adequate blue regions.

The standard light source for fluorescence microscopy is the Hg 100W ultra high pressure mercury lamp. The illumination optics of the incident light path in the microscope have been exactly matched to the size of the illuminated field of this lamp type, resulting in a significant improvement in the light flux and UV transmission.

The Hg 100W and the xenon 75W high-pressure lamp are run on stabilized direct current for extremely steady burning.

For routine fluorescence work in the laboratory, the more economical 50W Hg ultra high pressure lamp can be used if high luminosity is not required.