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Photomanipulation

The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells. By precisely adjusting sample labeling, biological activity, local chemical environments and in some instances, physically destroying parts of the specimen, researchers are able to access additional layers of information from their living cells.

Whether bleaching, activating, converting, ablating or combining techniques, researchers need to have a system fully capable of performing and capturing events in high resolution.

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Want to know more on our solutions for Photomanipulation?


Inverted microscope DMi8 with WF FRAP module
Inverted microscope DMi8 with WF FRAP module

Fluorescence Recovery After Photobleaching (FRAP)

Fluorescence Recovery After Photobleaching (FRAP) experiments require fast yet gentle and accurate bleaching of specific regions of interest (ROIs).

FRAP experiments typically are used in biological studies of cellular kinetics, such as membrane diffusion and protein binding.

Leica offers two solutions for FRAP experiments with the DMi8 inverted microscope

  • The WF FRAP module is a budget-friendly entry level FRAP all-in-one device.
  • For more advanced applications, use the Infinity Scanner module to perform multi-ROI high-speed photobleaching experiments.

Activating

Utilize the latest advances in photoswitchable fluorescent proteins.

By selectively activating regions of your cells, you can precisely follow how specific molecules behave in cells over time.

The Infinity Scanner gives you full flexibility to choose the wavelength, size, and shape of your activation regions.

Full integration into LAS X microscope software

With full integration into LAS X software, you can design and perform photoactivation experiments with the ease of a fully integrated system.

LAS X microscope software
LAS X microscope software

Cutting/Ablation

Perform laser ablation experiments by adding the Pulsed Laser Unit to the Infinity Scanner.

Ablation experiments require high power, precise cuts to specific structures, such as microtubules, or regions of cells in order to gain insights into structural and developmental processes.

Optogenetics

Using light to stimulate  conformational changes to proteins, optogenetic techniques offer researchers the ability to control specific changes in living cells and tissues. The versatile Infinity Scanner offers user the ability to perform optogenetics experiments, and to combine with additional photomanipulation techniques into one experiment.