LSF Germany - Prof. Dr. Paul M W French

Head of Photonics Group, Imperial College London (ICL)

“Multi-dimensional Fluorescence Imaging for Label-free Tissue Contrast, Molecular Cell Biology and High Content Analysis”

• Now online: Click here to watch the video

 

Abstract 

Fluorescence offers tremendous opportunities for molecular imaging using optical radiation but most fluorescence imaging is limited to recording fluorescence intensity to provide information concerning the localisation (distribution) of fluorescent labels – and the “target” proteins of interest to which they are attached.  

At Imperial we are developing technology to analyse fluorescence radiation with respect to wavelength, polarisation and, particularly, fluorescence lifetime, in order to maximise the information content of fluorescence imaging and provide enhanced molecular contrast.  This talk will review recent progress applying fluorescence lifetime imaging (FLIM) and multi-dimensional fluorescence imaging (MDFI) to tissue imaging and microscopy.