Prof. Marcel Bruchez, Carnegie Mellon University, Pittsburgh/PA at LSF France & Germany
May 26-28, 2010 - Advances in Life Science
May 26 - 28, 2010
“Genetically targeted multichromophore structures for bright, sensitive, and responsive cellular imaging"
- Venue
Bordeaux/FR, May 26th: 16h00 - 18h00
Université de Bordeaux, Institut François Magendie, Rue Camille Saint-Saens
Venue: Main Conference Room
Chair: Daniel Choquet
Paris/FR, May 27th: 16h00 - 18h00
Institut Curie, Developmental Biology Pôle, 26 rue d'Ulm, Paris
Venue: Main Conference Room
Chair: Jean Salamero
Heidelberg/DE, May 28th: 15h00 - 17h00
European Molecular Biology Laboratory (EMBL), Meyerhofstr., Heidelberg
Venue: Large Operon
Chair: Carsten Schultz
Agenda
Opening and Welcome
Lecture Marcel Bruchez
After Talk Reception & Meet the Speaker
Abstract
Fluorescent proteins and fluorescent dyes have become powerful tools in a range of live-cell and live-animal experiments.While intrinsically fluorescent proteins have outstanding properties for targeting, the brightness and functional reporter properties of these modules remain limited.
Organic dyes and quantum dots, on the other hand, are attractive because of high brightness, increased spectral ranges and targeted sensing functionality, although the targeting of these probes remains a challenge.
We have exploited noncovalent interactions between selected expressible polypeptides and low-fluorescence dye molecules to manipulate the brightness, achieving fluorescence enhancements in excess of 10,000-fold. These modules are tailored by modification of both the polypeptide and the dye molecule to provide unique sensing and imaging properties, reporting in real-time on subcellular location and target-specific physiology in fluorescent imaging at and beyond the optical diffraction limit.
- Followed by Discussion and Post Lecture Reception - Meet the Speaker
Registration
For organizational reasons, we would appreciate receiving your free registration by e-mail.
There will be a FREE bus shuttle from Bioquant (Im Neuenheimer Feld, Heidelberg) to EMBL and back. Please reserve your seat in advance. Contact: Sandra.Ebert@leica-microsystems.com
Event posters