Top: A nanoruler (such as DNA origami that has a defined 140 nm fluorophore spacing) is resolved with HyVolution, but not by traditional confocal imaging.
Bottom: Increase in axial resolution: Mitochondrial membranes labelled with TOM20-GFP. Sample courtesy Urs Ziegler, ZMB, University of Zurich, Switzerland

Confocal Super-Resolution Down to 140 nm

Resolving intracellular structures for a deeper insight in signaling networks can require resolution below 200 nm. That goes beyond the capability of a standard confocal microscope.

HyVolution provides confocal super-resolution down to 140 nm by combining confocal imaging with industry leading Huygens deconvolution by SVI. It even doubles the resolution in the axial direction.

The result: Your images show every detail with high fidelity

Upgrade any Leica TCS SP8 with HyVolution and also combine it with advanced imaging methods like multiphoton imaging and imaging with the White Light Laser.

Powered by SVI

Multiple Colors Simultaneously

HyVolution makes you see smaller details in multiple stained living cells.

Interactions of multiple proteins in fast dynamic processes become visible by true simultaneous multicolor imaging.

The filter-free spectral detection system of the Leica TCS SP8 records up to 5 different dye emissions coming from your specimen simultaneously.

That is outstanding, because single-detector arrangements lose time by sequential image acquisition.

HyVolution in multicolor live cells. All three colors were acquired simultaneously (no sequential scanning) in 5D. HeLa Kyoto cells. GPI-YFP (yellow), H2B-mCherry (blue), Tubulin-SiR (red). Sample courtesy of Sabine Reither, EMBL Heidelberg, Germany.
Multicolor acquisition of a Leica TCS SP8 with multiple super-sensitive HyDs compared to a single channel fiber-coupled GaAs PMT.

Multiple Colors in up to Five Channels Simultaneously

HyVolution allows multicolor acquisition with high speed. The filter-free spectral detection system of the Leica TCS SP8 can collect up to 5 simultaneous channels.

This allows much faster parallel data collection compared to single channel solution: You get 8 multicolor images with a Leica TCS SP8 with HyVolution in the same time that single-detector solutions take to provide only one single-color image.

No Compromise on Speed

Standard confocal systems sacrifice speed for resolution.

HyVolution does not limit the speed of image acquisition and also provides the full framerate of the Leica TCS SP8 (40 fps @ 512-512pixels) with multicolor super-resolution.

This allows you to capture events that occur within milliseconds and observe cellular dynamics in great detail and with unprecedented sharpness.

HyVolution in multicolor live cells. All three colors were acquired simultaneously (no sequential scanning) in 5D. HeLa Kyoto cells. Golgi-GFP(green), GPI-YFP (yellow), H2B-mCherry (white). Sample courtesy of Sabine Reither, EMBL Heidelberg, Germany.
Noise is detrimental for deconvolution results: the noisy image generated by a PMT is less likely to separate spots on a 140 nm DNA Origami nanoruler. Simulated data.

Deconvolution with HyD: Superior Reconstruction

Noise is detrimental for deconvolution. The background noise usually generated by PMTs is likely to produce more artefacts and less resolution.

The Leica TCS SP8 allows you to detect fluorescence even from weakly emitting samples virtually without background noise by:

  • Excellent signal-to-noise ratio of the photon-counting detector Leica HyD
  • High photon efficiency of the Leica TCS SP8

For you, this means less artefacts, unsurpassed imaging precision and confidence in what you publish.

HyVolution Wizard – Reproducible Results with Optimal Settings

The HyVolution Wizard integrated into the Leica Application Suite (LAS) X microscope software facilitates resolution-optimized imaging.

  1. You choose the level of resolution via a simple slider
  2. LAS X sees to all microscope settings, from pinhole size to the number of averaging
  3. Push a single button to exchange data between the Huygens deconvolution software and LAS X
  4. Directly compare the deconvolved images to the raw data and analyze them quantitatively with the LAS X software tools
Leica Application Suite (LAS) X Microscope Software

Parallel Architecture - GPU Accelerated

Deconvolution includes sophisticated and complex calculations.

This can be considerably speeded up by graphics processing unit (GPU) accelerated computing with the CUDA high-performance hardware by NVIDIA.

It dramatically increases computing performance by parallel processing of computationally intensive processes.

The result: You can access your data up to 10 times faster.