Live Cell Imaging Workshop at Icahn School of Medicine at Mount Sinai

Overview

Live Cell Imaging Workshop

Leica Microsystems is pleased to sponsor a live cell imaging workshop in conjunction with the Microscope Shared Imaging Facility at the Icahn School of Medicine at Mount Sinai. The workshop will focus on methods for imaging living samples and will be comprised of widefield, confocal, lightsheet and multiphoton systems.

Three days of seminars and hands-on practical sessions will be held from June 14-16, 2016.  Seminars are open to all and free to attend.  A small fee will be charged for hands-on sessions to support the cost of the event. Contact us for complete details.

Registration is required for hands-on sessions.  Please visit the Sign-up tab to register today.

This Microscopy course is brought to you in partnership with the Icahn School of Medicine at Mount Sinai:

Speakers

Speakers

Location: 5th Floor, Annenberg Seminar Room, Icahn School of Medicine at Mount Sinai
Dates: June 14-15, 2016

The last 10 minutes of each session will be saved for questions from the audience. Coffee and light refreshments will be provided.

June 14th:

  • 10-10:40 am: New Windows on Living Cells: Spatiotemporal dynamics of Rho GTPases regulate cancer invasion
    Dr. Louis Hodgson, Albert Einstein College of Medicine

  • 10:40-11:20 am: FRET and FLIM imaging
    Dr. Margarida Barroso, Albany Medical College

  • 11:20 am-12:10 pm: Keynote Seminar: Caveolae connect mechanical deformation to Calcium signals generated through G(alpha)q
    Dr. Suzanne Scarlata, Worcester Polytechnic Institute

  • 12:30-1:30 pm: Lunch

June 15th:

  • 10-10:30 am: The Molecular Choreography of Cell Division
    Dr. Julie Canman, Columbia University

  • 10:40-11:20 am: Signals and forces that control the dynamics of multicellular systems
    Dr. Jennifer Zallen, Memorial Sloan Kettering Cancer Center

  • 11:20 am-12:10 pm: Keynote Seminar: Experience-dependent dendritic spine plasticity in the cortex
    Dr. Wenbiao Gan, NYU Medical Center

  • 12:30-1:30 pm: Lunch

Sign-up: Hands-On Sessions

Hands-on Sessions

Location: Room S.203, 10th Floor, Hess Building, Icahn School of Medicine at Mount Sinai
Dates: June 14-16, 2016

Registration is required.

June 14th: Session 1

From 1:30 to 6:00 pm, there will be three rotations per session, 1.5 hours in length covering the following topics:

  • Microscope and Fluorescence Basics
  • Live Imaging on a Microscope
  • Live Cell Cinematography

June 15th: Session 2

From 1:30 to 6:00 pm, there will be three rotations per session, 1.5 hours in length covering the following topics:

  • Optical Sectioning with Confocal Microscopy
  • Fast, Dynamic, and Molecular Imaging including FRET and Ion Concentrations
  • Long Term Timelapse Imaging, Multi-position, Focus and Environment

June 16th: Session 3

From 9:30 am to 2:00 pm, there will be three rotations per session, 1.5 hours in length covering the following topics:

  • Photomanipulation
  • Live Data Analysis
  • Additional Topic TBD

In addition to the hands-on workshop, there will be two special sessions:

June 16th: Session 4

  • 2:30 pm: Digital Light Sheet

June 16th: Session 5

  • 3:30 pm: Multiphoton Imaging
Sign up Form - Hands-On Sessions
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System Details

Leica TCS SP8 with DLS
Digital Light Sheet Technology

With Digital Light Sheet (DLS) now on the Leica TCS SP8, you can benefit from two imaging methods on one system. DLS produces single plane illumination which reduces phototoxicity, and is ideal for rapid or long term imaging of organisms. And since DLS is on a confocal platform, you can use the imaging lasers for photomanipulation of the samples.

Learn more about the Leica TCS SP8 X at http://www.leica-microsystems.com/products/confocal-microscopes/details/product/leica-tcs-sp8-dls/.

Leica DMi8
Modular Inverted Microscope

As your research and lab grows, the Leica DMi8 is able to grow with you. Its unique flexible design allows you to configure and build a microscope that fits your needs and budget today, while offering upgradability for future research needs.

The modularity of the Leica DMi8 allows you to add:

  • Fluorescence
  • DIC
  • Automation
  • FRAP
  • Confocal capabilities
  • Third-party accessories including incubation, LED fluorescence, sCMOS cameras, and more

Learn more about the Leica DMi8 at http://www.leica-microsystems.com/products/light-microscopes/life-science-research/inverted-microscopes/the-leica-dmi8/.

Contacts