It is fully compatible with the reagent baths currently in use (16707154) and it is specially designed to accommodate all 6 mm carriers used for sample freezing in the Leica EM HPM100:
- 6mm sapphire discs (16770158) and
- 6 mm planchette carriers both the aluminum sample carriers (16770126 and16770127) and
- the copper-gold plated carriers (16770181 and 16770182).
Please note that in the case of manual filling, changing or discarding of fluids should be done only via the central inlet (marked with asterisk on the Fig.2). Overspill/contamination of the inner segment (arrow head) should be avoided as it will lead to poor polymerization of the resin.
Use fine forceps to carefully position the 6mm carriers (cells/sample facing up) in the individual baskets within the flow-through insert.
To avoid re-crystallization or drying of the samples make sure the insert is filled with 4 ml substitution medium, pre-cooled to the appropriate temperature, prior to loading the sample.
After polymerization is completed release the flow-through insert from the reagent bath. Make incisions with a razor blade (Fig.3) to enable the separation of the flow-through insert from the reagent bath.
Next, clean the bottom part of the flow-through insert from excess resin (Fig.4).
Remove the resin between the baskets (Fig.6).
Make an incision with a razor blade in the flow-through insert from both sides of the basket, as shown on figure 7 (to the right ). Do not cut completely through! The incision should be half the length of the basket.
With the help of forceps bend the resin block and release it from the basket (Fig.8).
Once the block is released from the mold, gently remove the fine layer of resin covering the sapphire disc (Fig.9).
Make sure there is no residual resin surrounding the rim of the sapphire and cool down the sapphire by dipping it shortly into