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Label-free FLIM: Microscopy under Physiological Conditions

Many biological samples exhibit autofluorescence. Its often broad spectra can interfere with fluorescent labeling strategies.

2D lifetime – wavelength spectrum. Sum of three autofluorescent components.

This application demonstrates how autofluorescence can serve as an intrinsic contrast in fluorescence lifetime imaging microscopy (FLIM) resulting in multi-color image stacks.

Read article: Label-free FLIM: Microscopy under Physiological Conditions

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