Precision-Cut Slices of Normal and Tumorous Liver Tissues
Generated with the Leica VT1200 S Vibrating Blade Microtome
Abstract
Precision-cut liver tissue slices are a powerful tool to perform studies on pharmacological metabolism as well as studies investigating toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been realized using different slicing machines. Since there has been great variability in the results, we sought to develop a highly reproducible method for slicing of liver tissue.
Liver samples from five different species (human, mouse, rat, pig, cattle) were cut using the Leica VT1200 S with Vibrocheck and the reproducibility of slice thickness and quality was determined. Compared to slicers used in the field so far, we demonstrated an improved accuracy and reproducibility of tissue slices.
Cow, 04; 20x