Professor of Bioengineering
Investigator, Howard Hughes Medical Institute
Stanford University, CA
An exciting emerging area revolves around the use of microfluidic tools for single-cell genomic analysis. We have been using microfluidic devices for both gene expression analysis and for genome sequencing from single cells. In the case of gene expression analysis, it has become routine to analyze hundreds of genes per cell on hundreds to thousands of single cells per experiment. This has led to many new insights into the heterogeneity of cell populations in human tissues, especially in the areas of cancer and stem cell biology.
These devices make it possible to perform “reverse tissue engineering” by dissecting complex tissues into their component cell populations, and they are also used to analyze rare cells such as circulating tumor cells or minor populations within a tissue.
We have also used single-cell genome sequencing to analyze the genetic properties of microbes that cannot be grown in culture—the largest component of biological diversity on the planet—as well as to study the recombination potential of humans by characterizing the diversity of novel genomes found in the sperm of an individual.
We expect that single cell genome sequencing will become a valuable tool in understanding genetic diversity in many different contexts.
Leica Scientific Forum Berlin
November 13, 2013 –16:00, Chair: Prof. Stephan Sigrist
Venue: Hörsaal 2005, EG Rundbau, Konrad-Zuse-Zentrum für Informationstechnik Berlin (ZIB), Takustraße 7, 14195 Berlin-Dahlem
Leica Scientific Forum Heidelberg
November 14, 2013 – 16:00h, Chair: Dr. Rainer Pepperkok
Venue: Klaus Tschira Auditorium at EMBL Advanced Training Center, Meyerhofstraße 1, 69117 Heidelberg
--> There will be free a bus shuttle from Neuenheimer Feld to EMBL & back. Please contact Sandra Ebert for details: LSF[at]leica-microsystems.com / phone: 06441 292528
Leica Scientific Forum Munich / CeNS Colloquium
November 15, 2013 – 16:15h, Chair: Prof. Hermann Gaub
Venue: Raum H030, Fakultät für Physik, Schellingstraße 4, 80799 München
All lectures are free of charge. For organizational reasons, please kindly RSVP by e-mail.
The members of the Scientific Advisory Board Germany are:
Berlin: Prof. Michael Brecht (HUB), Prof. Stephan Sigrist (FUB) - Heidelberg: Prof. Winfried Denk (MPI), Prof. Roland Eils (DKFZ), Prof. Stefan Hell (DKFZ and MPI Göttingen), Dr. Rainer Pepperkok (EMBL) - Munich: Prof. Ulrike Gaul (LMU), Prof. Hermann Gaub (LMU), Prof. Arthur Konnerth (TUM) & Dr. Thomas Zapf (Leica Microsystems)