The Future of STED Microscopy Has Only Just Begun
Perspectives of Superresolution in Neurobiology
Contact and Information
Wernher Fouquet, PhD, NeuroCure, AG Sigrist
wernher.fouquet@charite.de
http://genetik.bcp.fu-berlin.de/
Image Gallery
Images: © W. Fouquet
- 1: Presynaptic T-bar structure depicted by different imaging techniques. (A) NMJ labeled with two antibodies for BRP, either for the C-term (Nc82) or N-term. Because of its spherical structure of single subdivisions of the Drosophila NMJ (boutons), single synapses (see inlet) show a segregation of the BRPN-Term label towards the outside of the bouton. As in these regions the synaptic membrane lies perpendicular to the focal plane, a polarized orientation of BRP from the membrane to cytoplasm may be implicated. (B) Same structure now imaged with STED microscopy (green) and a confocal reference (red). In these images an architectural arrangement was described for BRP that depicts striking similarities to the T-bar observed in electron micrographs (C). Images taken with a Leica TCS STED.
- 2: Live cell imaging with the Leica TCS STED CW. Consecutive STED images (single confocal slices) of CitrineDLiprin-α in intact living Drosophila larvae demonstrate, in principle, the possibilities for time lapse and FRAP experiments.
