Routine & Special Staining
Routine (H&E) staining is the corner stone of tissue-based diagnosis. The process stains thin tissue sections so that pathologists can visualize tissue morphology. The process uses a haematoxylin dye to stain cell nuclei (and other parts) blue and an eosin dye to stain other structures pink or red. Properly applied, this technique provides exceptional detail of tissue structure and the makeup of the cells. This detail is required for tissue-based diagnosis, particularly in the detection and classification of cancer. Special stains use a variety of dyes and techniques to stain particular tissues, structures or pathogens (such as bacteria) to assist pathologists with tissue-based diagnosis.
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Article
05. May 2011 Institute: Leica Biosystems, Wetzlar, GermanyStaining protocols utilizing hematoxylin are the most commonly used of the routine staining procedures performed in the histology laboratory. This article is a review of the current state of understanding of the chemistry of aluminum based hematoxylin stains and the mechanism by which these stains… Read article
Events
Useful Links
Human Histology & Introductory Pathology
histology.leeds.ac.uk/what-is-histology/
Basic introduction to histology
www.pathologyoutlines.com/
Comprehensive textbook of surgical and clinical pathology
pathmd.com
Educational tools for pathology residents and physicians
www.path.uiowa.edu/virtualslidebox/
Virtuel Slidebox for histology and histopathology
teaching.anhb.uwa.edu.au/mb140/
Blue histology - broad coverage of histology
library.med.utah.edu/
Health science library
Histotechnology
stainsfile.info/StainsFile/jindex.html
Useful information about dyes, stains and staining
www.ihcworld.com/index.htm
Information Source for immunohistochemistry
www.bristol.ac.uk/vetpath/cpl/histmeth.htm
Useful information on staining techniques
Organizations and Discussion Groups
www.histonet.org/
Histonet - listserver for the histology profession
www.nsh.org/
The National Society for Histotechnology
www.hgv.org.au/
The Histology Group of Victoria
www.hgq.org.au/
Histotechnology Group of Queensland
www.histonsw.org.au/
Histotechnology Group of NSW
Quality Assessment Programs
www.ukneqas.org.uk
United Kingdom National External Quality Assessment Service
www.nordiqc.org/
Nordic Immunohistochemical Quality Group
www.rcpaqap.com.au/
The Royal College of Pathologists of Australia
www.nsh.org/content/all-about-histoqip
HistoQIP quality assurance program
Search Engines
www.histosearch.com/
Search engine for histology information
www.antibodybeyond.com/
Comprehensive information source about antibodies and IHC
www.gopubmed.com
Search interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_engines
List of academic databases and search engines
scholar.google.com
Beta of Google's search engine for scientific abstracts
Journals
jcp.bmj.com/
Journal of Clinical Pathology
www.pathologyinpractice.com/
Pathology in Practice - practical solutions, news, case studies
www.doaj.org/
Directory of open access journals
www.sciencemag.org/
Science
www.nature.com/
Nature
www.plosone.org/home.action
International, peer-reviewed, open-access online publication
Useful Text Books
Human Histology & Introductory Pathology
Young B, O’Dowd G, Stewart W, (2010): Wheater's Basic Pathology: A Text, Atlas and Review of Histopathology (5th ed.)
Kerr JB. (2010): Functional Histology (2nd ed.)
Mescher AL. (2009): Jun queira's Basic Histology ( 12th ed.)
Eroschenko VP. (2007): diFiore's Atlas of Histology with Functional Correlations (11th ed.)
Young B, Lowe JS, Stevens A, Heath JW (2006): Wheater's Functional Histology (5th ed.)
Histotechnology
Carson F, Hladik C. (2009): Histotechnology: A Self-Instructional Text. (3rd ed.)
Kiernan J. (2008): Histological and Histochemical Methods: Theory and Practice. (4th ed.)
Bancroft JD, Gamble M. (2007). Theory and Practice of Histological Techniques. (6 th ed.)
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