Enjoy spectral freedom with 4Tune
The number of transgenic markers evolves constantly and rapidly. The SP8 DIVE keeps apace with these developments and can adapt to existing and new transgenic markers with just a few mouse clicks.
Spectral freedom results from the new groundbreaking 4Tune technology for non-descanned detection. The 4Tune detector allows you to adapt to the full emission and to separate strongly overlapping spectra – without mathematical restoration.
The SP8 DIVE enables you to capture twice as much of the fluorescence signal, which translates into improved penetration depth, imaging speed, or less phototoxicity for in vivo imaging.
"The conventional dichroics are never optimal to distinguish all fluorophores, but with the spectral detectors this is now possible and much easier, since we can really optimize the wavelength for each fluorophore you want to detect."
Prof. Dr. Jacco van Rheenen. University of Utrecht, the Netherlands
Istituto Italiano di Tecnologia (IIT), Genua
“Great for further disseminating multiphoton microscopy, today. It is a big jump related to the increased range of applications and the solution of a lot of problems related to the variety of samples. It is worth noting the integration of SHG and two-photon images in an effective way.
I think that the possibility of coupling with other Leica solutions and products, including STED modules, will make this architecture tunable for the different experimental needs from spatial to temporal resolution to forthcoming fluorophores and methods.
I love the possibility of controlling and visualizing the filling of the back focal plane enlarging or shrinking the two-photon excitation illumination beam. This is great.”
Ingénieur de Recherche
INSERM, Centre de physiopathologie de Toulouse Purpan
“Its amazing because of the modularity of the features and because the use of filter cubes in front of NDD detectors impact on the choice of fluorochromes, and sometimes we are obliged to adapt the sample preparation and change fluorochromes. Now it will be much easier.
No, I am convinced. I am doing two-photons for 10 years now, four colors and so its very interesting.”
Netherlands Cancer Institute
"I just got a demo and I am flabbergasted. I think its absolutely marvelous and you bring anything we had for visible light now to the infrared. You did a really great job."
LCAM, van Leeuwenhoek Centre for Advanced Microscopy, University Amsterdam
“I like the new dual photon spectral detector. What I like most is how it is embedded in the software.
I am managing an imaging center, and the easier it is for the user, the better. We have 200+ users and training them on very complicated machines takes its time.
So the easier it is , the more intuitive it is, the better.”
W.M. Keck Center for Cellular Imaging, VA
“I like the wavelengths setting from 740 to 920. That is a very good option of what can use. I mean how many wavelengths they want to use depending on the fluorophore. And yes, if they use one wavelength and then have two or three detectors to play around and tune, a lot of options.
The signal to noise ration is very good, flexibility on the emission side.
There is no doubt, it is very important to see the user friendliness of the system. So that’s what I am looking for. Since I work with Leica, I follow the user friendliness of these operation of the two photon operation vs. one photon operations."
[During this demonstration at the Focus on Microscopy 2017, data of 4 labels were sequentially acquired using the SP8 DIVE equipped with one