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Confetti Mouse Small Intestine. CFP, GFP, YFP and RFP
Sample courtesy of Jacco van Rheenen, University of Utrecht, the Netherlands

Enjoy complete multispectral freedom

The SP8 DIVE comes with a unique non-descanned detection system. Detect up to four colors simultaneously or an infinite number of colors sequentially within the spectral range from 380 to 800 nm.

Track sub-cellular actions in realtime with all current and future markers. Colocalize multiple proteins in deep tissue and track a multitude of cell types in vivo.

With SP8 DIVE you are prepared for experiments with every existing and future transgenic marker!

"The conventional dichroics are never optimal to distinguish all fluorophores, but with the spectral detectors this is now possible and much easier, since we can really optimize the wavelength for each fluorophore you want to detect."

Prof. Dr. Jacco van Rheenen. University of Utrecht, the Netherlands

Alberto Diaspro

Istituto Italiano di Tecnologia (IIT), Genua

“Great for further disseminating multiphoton microscopy, today. It is a big jump related to the increased range  of applications and the solution of a lot of problems related to the variety of samples. It is worth noting the integration of SHG and two-photon images in an effective way.

I think that the possibility of coupling with other Leica solutions and products, including STED modules, will make this architecture tunable for the different experimental needs from spatial to temporal resolution to forthcoming fluorophores and methods.  

I love the possibility of controlling and visualizing the filling of the back focal plane enlarging or shrinking the two-photon excitation illumination beam. This is great.”

Sophie Allart

Ingénieur de Recherche
INSERM, Centre de physiopathologie de Toulouse Purpan

“Its amazing because of the modularity of the features and because the use of filter cubes in front of NDD detectors impact on the choice of fluorochromes, and sometimes we are obliged to adapt the sample preparation and change fluorochromes. Now it will be much easier.

No, I am convinced. I am doing two-photons for 10 years now, four colors and so its very interesting.”

Kees Jalink

Netherlands Cancer Institute

"I just got a demo and I am flabbergasted. I think its absolutely marvelous and you bring anything we had for visible light you now have infrared. You did a really great job."

Ron Hoebe

LCAM, van Leeuwenhoek Centre for Advanced Microscopy, University Amsterdam

“I like the new dual photon spectral detector. What I like most is how it is embedded in the software.

I am managing an imaging center, and the easier it is for the user, the better. We have 200+ users and training them on very complicated machines takes its time.

So the easier it is , the more intuitive it is, the better.”

Ammasi Periasami

W.M. Keck Center for Cellular Imaging, VA

“I like the wavelengths setting from 740 to 920. That is a very good option of what can use. I mean how many wavelengths they want to use depending on the fluorophore. And yes, if they use one wavelength and then have two or three detectors to play around and tune, a lot of options.

The signal to noise ration is very good, flexibility on the emission side.

There is no doubt, it is very important to see the user friendliness of the system. So that’s what I am looking for. Since I work with Leica, I follow the user friendliness of these operation of the two photon operation vs. one photon operations."

Marc van Zandvoort

Maastricht University, Genetics and Cell Biology

“Very nice extension of the current existing two photon systems. This extension makes the system much more flexible in use of the two photon direct detectors.

So far, that use was very limited, due to the need of separate filters. In that sense its a very good extension.”

Urs Ziegler

Head of Facility, Center for Microscopy and Image Analysis, University of Zurich

"It’s very flexible and very easy to use. You don’t have to manually change filters and you can use all the dyes you can think of.
What I appreciate is that, basically, in the software you don’t have to train someone who already knows confocal scanning microscopy. You don’t have to manually change filters – DIVE is easy to use because it takes care of the different combinations of filters and dyes, which although possible, is not so easy to do. So this is already a major advantage.

And then obviously, in live tissue you have various dyes you can use, second and third harmonics. Using SP8 DIVE this can be done very, very easily. I think this is a major step forward."

DIVE with ease – the 4Tune detector

The number of transgenic markers evolve constantly and rapidly.  The SP8 DIVE keeps apace with developments and can adapt to existing and new transgenic marker with just a few mouse clicks.

Such spectral freedom results from the new groundbreaking 4Tune technology for non-descanned detection. 4Tune enables the spectral characteristics of up to four bands to be quickly and precisely tuned. An extensive library of fluorescence spectra acts as a guide to optimize the setup.

SP8 DIVE enables researchers to capture up to 67% more of the fluorescence signal from the investigated marker signal and, as a result, achieve more contrast and depth for multi-color in vivo deep Imaging.

Inflexible hardware filters, long setup times, and limited ability to adapt experiments are no longer a limitation.

Top graph: Rapid increase of markers
Bottom: 4Tune user interface
Maximum resolution (left) & maximum depth (right)

Explore new dimensions in depth

With SP8 DIVE you can tune for deepest insight or finest detail. The beams can be optimally adjusted for each objective with the new Vario Beam Expander (VBE).

The VBE allows for optimized co-localization and the right balance of resolution and depth in line with the research question.

Multispectral in vivo imaging is available for up to 3 excitation lines and our full IR objective range.

The Vario Beam Expander (VBE) 

  • Tune for deepest insight or finest detail
  • Optimally adjust the beam for each objective
  • Tune for perfect colocalization
  • Precisely control the position of photomanipulation volume
Tunable Vario Beam Expander (VBE)
top: Versatile platform SP8 (left), Nanoscopy (middle), Super-Resolution (right)
bottom: CARS (left), Light Sheet (middle), SMD (right)

DIVE - a member of the SP8 platform

The SP8 DIVE is the latest addition to the established SP8 platform from Leica. The platform can be configured to accommodate different research methods ranging from confocal microscopy with Super-Resolution to STED Nanoscopy.

For users, this delivers both versatility and investment protection.

All instruments of the SP8 platform are open and adaptable to the user’s research, both now and in the future.