Images Leica TCS SP5 MP

Energy diagram
Energy diagram of fluorescence with 1-photon and 2-photon excitation

Plathynereis
Plathynereis spec.
Courtesy of Dr. Leonid Nelszin, RSA, Moscow, Russia

Zebrafish
Dorsal musculature of zebrafish

FLIM with MP excitation
Zebrafish image (bottom) and corresponding intensity image (top) with nuclei, muscle tissue and yolk. The histogram (right) contains the legend for lifetimes.