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Leica TCS SP8 STED

STED Nanoscopes Leica TCS SP8 STED

It is already 20 years ago that STED microscopy was first described by Stephan Hell. In the meantime it has become an important tool to study subcellular structures beyond the diffraction limit. This reference list gives an overview about the technological development of STED nanoscopy and also shows selected publications performed on a Leica STED instrument.

STED Key Publications

First publication on STED technology:

Hell, S. W. & Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics letters 19, 780-782, (1994). http://www.ncbi.nlm.nih.gov/pubmed/19844443

First biological image:

Klar, T. A., Jakobs, S., Dyba, M., Egner, A. & Hell, S. W. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proceedings of the National Academy of Sciences of the United States of America 97, 8206-8210, (2000). http://www.ncbi.nlm.nih.gov/pubmed/10899992

STED with genetically encoded marker: GFP

Willig, K. I. et al. Nanoscale resolution in GFP-based microscopy. Nature methods 3, 721-723, (2006). http://www.ncbi.nlm.nih.gov/pubmed/16896340

STED with continuous wave laser beams:

Willig, K. I., Harke, B., Medda, R. & Hell, S. W. STED microscopy with continuous wave beams. Nature methods 4, 915-918, (2007). http://www.ncbi.nlm.nih.gov/pubmed/17952088

Two-color STED with two STED lines

Donnert, G. et al. Two-color far-field fluorescence nanoscopy. Biophysical journal 92, L67-69, (2007). http://www.ncbi.nlm.nih.gov/pubmed/17307826

Iso-STED and two-color STED with one STED line

Schmidt, R. et al. Spherical nanosized focal spot unravels the interior of cells. Nature methods 5, 539-544, (2008). http://www.ncbi.nlm.nih.gov/pubmed/18488034

STED- FCS application

Eggeling, C. et al. Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature 457, 1159-1162, (2009). http://www.ncbi.nlm.nih.gov/pubmed/19098897

Gated STED

Vicidomini, G. et al. Sharper low-power STED nanoscopy by time gating. Nature methods 8, 571-573, (2011). http://www.ncbi.nlm.nih.gov/pubmed/21642963

 

Selected Publications with Leica STED instrument

2016

Agostinho, A. et al. High density of REC8 constrains sister chromatid axes and prevents illegitimate synaptonemal complex formation. EMBO Rep. 17, 901-913, (2016). http://EMBOr.embopress.org/cgi/content/abstract/17/6/901

Aix, E., Gutierrez-Gutierrez, O., Sanchez-Ferrer, C., Aguado, T. & Flores, I. Postnatal telomere dysfunction induces cardiomyocyte cell-cycle arrest through p21 activation. J. Cell Biol. 213, 571-583, (2016). http://jcb.rupress.org/cgi/content/abstract/213/5/571

Brandenburg, S. et al. Axial tubule junctions control rapid calcium signaling in atria. The Journal of Clinical Investigation 126, 3999-4015, (2016). https://doi.org/10.1172/JCI88241

Clausen, M. P. et al. A straightforward approach for gated STED- FCS to investigate lipid membrane dynamics. Methods 88, 67-75, (2015). http://www.sciencedirect.com/science/article/pii/S1046202315300050

Coto Hernández, I. et al. Two-Photon Excitation STED Microscopy with Time-Gated Detection. Scientific Reports 6, 19419, (2016). http://dx.doi.org/10.1038/srep19419

Daubon, T., Spuul, P., Alonso, F., Fremaux, I. & Genot, E. VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells. J. Cell Sci. 129, 2586-2598, (2016). http://jcs.biologists.org/cgi/content/abstract/129/13/2586

Grauel, M. K. et al. RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses. PNAS 113, 11615-11620, (2016). http://www.pnas.org/cgi/content/abstract/113/41/11615

Henkel, B., Bintig, W., Bhat, S. S., Spehr, M. & Neuhaus, E. M. NHERF1 in Microvilli of Vomeronasal Sensory Neurons. Chem Senses, bjw094, (2016). http://chemse.oxfordjournals.org/cgi/content/abstract/bjw094v1

Inaba, Y. et al. Soluble bioactive microbial mediators regulate proteasomal degradation and autophagy to protect against inflammation-induced stress. Am J Physiol Gastrointest Liver Physiol 311, G634-647, (2016). http://ajpgi.physiology.org/cgi/content/abstract/311/4/G634

Jankovicova, J. et al. Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes. Reproduction 152, 785-793, (2016). http://www.reproduction-online.org/cgi/content/abstract/152/6/785

Ke, M. T. et al. Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent. Cell Rep 14, 2718-2732, (2016). https://www.ncbi.nlm.nih.gov/pubmed/26972009

Kulibin, A. Y. & Malolina, E. A. Only a small population of adult Sertoli cells actively proliferates in culture. Reproduction 152, 271-281, (2016). http://www.reproduction-online.org/cgi/content/abstract/152/4/271

Lee, D.-K. et al. Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance. Plant Physiology 172, 575-588, (2016). http://www.plantphysiol.org/cgi/content/abstract/172/1/575

Lee, H. et al. Cln 3-requiring 9 is a negative regulator of Th17 pathway-driven inflammation in anti-glomerular basement membrane glomerulonephritis. Am J Physiol Renal Physiol 311, F505-519, (2016). http://ajprenal.physiology.org/cgi/content/abstract/311/3/F505

Li, Q. et al. A pair of transposon-derived proteins regulate active DNA demethylation in Arabidopsis. EMBO J., embj.201694284, (2016). http://EMBOJ.embopress.org/cgi/content/abstract/embj.201694284v1

Liu, Q. et al. MICAL3 Flavoprotein Monooxygenase Forms a Complex with Centralspindlin and Regulates Cytokinesis. J. Biol. Chem. 291, 20617-20629, (2016). http://www.jbc.org/cgi/content/abstract/291/39/20617

Lopez de Lapuente, A. et al. Novel Insights into the Multiple Sclerosis Risk Gene ANKRD55. J. Immunol. 196, 4553-4565, (2016). http://www.jimmunol.org/cgi/content/abstract/196/11/4553

Marcos-Ramiro, B. et al. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border. J. Cell Biol. 213, 385-402, (2016). http://jcb.rupress.org/cgi/content/abstract/213/3/385

Medda, R., Giske, A. & Cavalcanti-Adam, E. A. Challenges in imaging cell surface receptor clusters. Optics and Lasers in Engineering 76, 3-8, (2016). http://www.sciencedirect.com/science/article/pii/S0143816615000688

Morelli, E. et al. An essential step of kinetochore formation controlled by the SNARE protein Snap29. EMBO J. 35, 2223-2237, (2016). http://EMBOJ.embopress.org/cgi/content/abstract/35/20/2223

Nakayama, H. et al. Lipoarabinomannan binding to lactosylceramide in lipid rafts is essential for the phagocytosis of mycobacteria by human neutrophils. Sci. Signal. 9, ra101-, (2016). http://stke.sciencemag.org/cgi/content/abstract/sigtrans;9/449/ra101

Nejedla, M. et al. Profilin connects actin assembly with microtubule dynamics. Mol. Biol. Cell 27, 2381-2393, (2016). http://www.molbiolcell.org/cgi/content/abstract/27/15/2381

Noordstra, I. et al. Control of apico-basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7. J. Cell Sci. 129, 4278-4288, (2016). http://jcs.biologists.org/cgi/content/abstract/129/22/4278

Pageon, S. V. et al. Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination. PNAS 113, E5454-5463, (2016). http://www.pnas.org/cgi/content/abstract/113/37/E5454

Roussa, E. et al. The membrane trafficking and functionality of the K+-Cl- co-transporter KCC2 is regulated y TGF-{beta}2. J. Cell Sci. 129, 3485-3498, (2016). http://jcs.biologists.org/cgi/content/abstract/129/18/3485

Samson, A. L. et al. Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells. Open Bio 6, 160098-, (2016). http://rsob.royalsocietypublishing.org/cgi/content/abstract/6/11/160098

Schedin-Weiss, S., Caesar, I., Winblad, B., Blom, H. & Tjernberg, L. O. Super-resolution microscopy reveals γ-secretase at both sides of the neuronal synapse. Acta Neuropathologica Communications 4, 1-12, (2016). http://dx.doi.org/10.1186/s40478-016-0296-5

Scheuring, D. et al. Actin-dependent vacuolar occupancy of the cell determines auxin-induced growth repression. PNAS 113, 452-457, (2016). http://www.pnas.org/cgi/content/abstract/113/2/452

Sezgin, E. et al. A comparative study on fluorescent cholesterol analogs as versatile cellular reporters. J. Lipid Res. 57, 299-309, (2016). http://www.jlr.org/cgi/content/abstract/57/2/299

Sneddon, W. B. et al. Convergent Signaling Pathways Regulate Parathyroid Hormone and Fibroblast Growth Factor-23 Action on NPT2A-mediated Phosphate Transport. J. Biol. Chem. 291, 18632-18642, (2016). http://www.jbc.org/cgi/content/abstract/291/36/18632

Sosa-Costa, A. et al. Lateral Mobility and Nanoscale Spatial Arrangement of Chemokine-activated {alpha}4{beta}1 Integrins on T Cells. J. Biol. Chem. 291, 21053-21062, (2016). http://www.jbc.org/cgi/content/abstract/291/40/21053

Torreno-Pina, J. A. et al. The actin cytoskeleton modulates the activation of iNKT cells by segregating CD1d nanoclusters on antigen-presenting cells. PNAS 113, E772-781, (2016). http://www.pnas.org/cgi/content/abstract/113/6/E772

Toya, M. et al. CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells. PNAS 113, 332-337, (2016). http://www.pnas.org/cgi/content/abstract/113/2/332

Unnersjö-Jess, D., Scott, L., Blom, H. & Brismar, H. Super-resolution stimulated emission depletion imaging of slit diaphragm proteins in optically cleared kidney tissue. Kidney International 89, 243-247, (2016). http://www.sciencedirect.com/science/article/pii/S0085253815000356

Wegel, E. et al. Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison. Sci Rep 6, 27290, (2016). https://www.ncbi.nlm.nih.gov/pubmed/27264341

Wojtuszkiewicz, A. et al. Exosomes secreted by apoptosis-resistant AML blasts harbor regulatory network proteins potentially involved in antagonism of apoptosis. Molecular & Cellular Proteomics, mcp. M115. 052944, (2016)

Yang, X. et al. Mirror-enhanced super-resolution microscopy. Light Sci Appl. 5, e16134, (2016). http://dx.doi.org/10.1038/lsa.2016.134

Yonemura, Y. et al. Inhibition of cargo export at ER exit sites and the trans-Golgi network by the secretion inhibitor FLI-06. J. Cell Sci. 129, 3868-3877, (2016). http://jcs.biologists.org/cgi/content/abstract/129/20/3868

 

 

 

2015

 

Beckmann, H. et al. Spectral sensitivity in Onychophora (velvet worms) revealed by electroretinograms, phototactic behaviour and opsin gene expression. J. Exp. Biol. 218, 915-922, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25617459

Benard, M. et al. Structural and functional analysis of tunneling nanotubes (TnTs) using gCW STED and gconfocal approaches. Biol. Cell., (2015). http://www.ncbi.nlm.nih.gov/pubmed/26094971

Boyce, A. K., Kim, M. S., Wicki-Stordeur, L. E. & Swayne, L. A. ATP stimulates pannexin 1 internalization to endosomal compartments. Biochem. J. 470, 319-330, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26195825

Cao, J. et al. Circovirus transport proceeds via direct interaction of the cytoplasmic dynein IC1 subunit with the viral capsid protein. J. Virol. 89, 2777-2791, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25540360

Chen, A. L. et al. Novel components of the Toxoplasma inner membrane complex revealed by BioID. mBio 6, e02357-02314, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25691595

Chen, L., Xie, Z., Turkson, S. & Zhuang, X. A53T human alpha-synuclein overexpression in transgenic mice induces pervasive mitochondria macroautophagy defects preceding dopamine neuron degeneration. The Journal of neuroscience : the official journal of the Society for Neuroscience 35, 890-905, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25609609

Clausen, M. P. et al. A straightforward approach for gated STED- FCS to investigate lipid membrane dynamics. Methods, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26123184

Depreux, F. F. et al. Disruption of the lamin A and matrin-3 interaction by myopathic LMNA mutations. Hum. Mol. Genet., ddv160, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25948554

Glancy, B. et al. Mitochondrial reticulum for cellular energy distribution in muscle. Nature 523, 617-620, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26223627

Hayashi, S. & Okada, Y. Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics. Mol. Biol. Cell 26, 1743-1751, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25717185

Henkel, B. et al. Co-expression of anoctamins in cilia of olfactory sensory neurons. Chem. Senses 40, 73-87, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25500808

Hirsch, M. & Helm, M. Live cell imaging of duplex siRNA intracellular trafficking. Nucleic Acids Res. 43, 4650-4660, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25870407

Hoepker, A. C. et al. Genetically encoded sensors of protein hydrodynamics and molecular proximity. Proceedings of the National Academy of Sciences of the United States of America 112, E2569-2574, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25931526

Ito, C., Yamatoya, K. & Toshimori, K. Analysis of the complexity of the sperm acrosomal membrane by super-resolution stimulated emission depletion microscopy compared with transmission electron microscopy. Microscopy (Oxford, England) 64, 279-287, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25430742

Ivanova, D. et al. Synaptic activity controls localization and function of CtBP1 via binding to Bassoon and Piccolo. The EMBO journal 34, 1056-1077, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25652077

Iwabuchi, K. et al. Properties and functions of lactosylceramide from mouse neutrophils. Glycobiology 25, 655-668, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25595946

Jiang, M. et al. Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis. The Journal of cell biology 210, 595-611, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26261180

Johnson, A. et al. TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization. The EMBO journal 34, 811-827, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25586378

Jung, S. et al. Disruption of adaptor protein 2mu (AP-2mu) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing. The EMBO journal 34, 2686-2702, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26446278

Jung, S. et al. Rab3-interacting molecules 2alpha and 2beta promote the abundance of voltage-gated CaV1.3 Ca2+ channels at hair cell active zones. Proceedings of the National Academy of Sciences of the United States of America 112, E3141-3149, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26034270

Lagrue, K., Carisey, A., Morgan, D. J., Chopra, R. & Davis, D. M. Lenalidomide augments actin remodeling and lowers NK-cell activation thresholds. Blood 126, 50-60, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26002964

Liszewski Zilla, M., Chan, Y. G., Lunderberg, J. M., Schneewind, O. & Missiakas, D. LytR-CpsA-Psr enzymes as determinants of Bacillus anthracis secondary cell wall polysaccharide assembly. Journal of bacteriology 197, 343-353, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25384480

Lunderberg, J. M., Liszewski Zilla, M., Missiakas, D. & Schneewind, O. Bacillus anthracis tagO Is Required for Vegetative Growth and Secondary Cell Wall Polysaccharide Synthesis. Journal of bacteriology 197, 3511-3520, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26324447

Meyer, T. et al. CD32a antibodies induce thrombocytopenia and type II hypersensitivity reactions in FCGR2A mice. Blood 126, 2230-2238, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26396093

Oh, D., Lee, S., Koo, K.-I. & Seo, J.-M. Microscopic Tubular Cell Organization for Artificial Vascularization. 6th European Conference of the International Federation for Medical and Biological Engineering 45, 322-325, (2015). http://dx.doi.org/10.1007/978-3-319-11128-5_80

Perrault, I. et al. IFT81, encoding an IFT-B core protein, as a very rare cause of a ciliopathy phenotype. J. Med. Genet. 52, 657-665, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26275418

Rubbini, D., Robert-Moreno, A., Hoijman, E. & Alsina, B. Retinoic Acid Signaling Mediates Hair Cell Regeneration by Repressing p27kip and sox2 in Supporting Cells. The Journal of neuroscience : the official journal of the Society for Neuroscience 35, 15752-15766, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26609166

Schachtrup, C. et al. Nuclear pore complex remodeling by p75NTR cleavage controls TGF-[beta] signaling and astrocyte functions. Nature neuroscience 18, 1077-1080, (2015). http://dx.doi.org/10.1038/nn.4054

Schuhmacher, J. S. et al. MinD-like ATPase FlhG effects location and number of bacterial flagella during C-ring assembly. Proceedings of the National Academy of Sciences of the United States of America 112, 3092-3097, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25733861

Strippoli, R. et al. Caveolin-1 deficiency induces a MEK-ERK1/2-Snail-1-dependent epithelial-mesenchymal transition and fibrosis during peritoneal dialysis. EMBO Mol. Med. 7, 102-123, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25550395

Stritt, S. et al. Rap1-GTP-interacting adaptor molecule (RIAM) is dispensable for platelet integrin activation and function in mice. Blood 125, 219-222, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25336629

Sun, N. et al. Measuring In Vivo Mitophagy. Mol. Cell, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26549682

Sundaravel, S. et al. Reduced DOCK4 expression leads to erythroid dysplasia in myelodysplastic syndromes. Proceedings of the National Academy of Sciences of the United States of America 112, E6359-6368, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26578796

Unnersjo-Jess, D., Scott, L., Blom, H. & Brismar, H. Super-resolution stimulated emission depletion imaging of slit diaphragm proteins in optically cleared kidney tissue. Kidney Int., (2015). http://www.ncbi.nlm.nih.gov/pubmed/26444032

Viero, G. et al. Three distinct ribosome assemblies modulated by translation are the building blocks of polysomes. The Journal of cell biology 208, 581-596, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25713412

Virtakoivu, R. et al. Vimentin-ERK Signaling Uncouples Slug Gene Regulatory Function. Cancer Res. 75, 2349-2362, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25855378

Wang, Y. et al. Overexpression of the Insulin-Like Growth Factor II Receptor Increases beta-Amyloid Production and Affects Cell Viability. Mol. Cell. Biol. 35, 2368-2384, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25939386

Yu, P. et al. Cooperative interactions of LPPR/PRG family members in membrane localization and alteration of cellular morphology. J. Cell Sci., (2015). http://www.ncbi.nlm.nih.gov/pubmed/26183180

Yu, P. et al. Cooperative interactions of LPPR family members in membrane localization and alteration of cellular morphology. J. Cell Sci. 128, 3210-3222, (2015). http://www.ncbi.nlm.nih.gov/pubmed/26183180

Zecca, A., Dyballa, S., Voltes, A., Bradley, R. & Pujades, C. The Order and Place of Neuronal Differentiation Establish the Topography of Sensory Projections and the Entry Points within the Hindbrain. The Journal of neuroscience : the official journal of the Society for Neuroscience 35, 7475-7486, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25972174

Zhang, Y., Moeini-Naghani, I., Bai, J., Santos-Sacchi, J. & Navaratnam, D. S. Tyrosine motifs are required for prestin basolateral membrane targeting. Biology open 4, 197-205, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25596279

Zheng, P., Bertolet, G., Chen, Y., Huang, S. & Liu, D. Super-resolution imaging of the natural killer cell immunological synapse on a glass-supported planar lipid bilayer. Journal of visualized experiments : JoVE, e52502, (2015). http://www.ncbi.nlm.nih.gov/pubmed/25741636

 

2014

Aljada, A., Saleh, A. M. & Al Suwaidan, S. Modulation of insulin/IGFs pathways by sirtuin-7 inhibition in drug-induced chemoreistance. Diagn. Pathol. 9, 94, (2014). http://www.ncbi.nlm.nih.gov/pubmed/2488596

Andlauer, T. F. et al. Drep-2 is a novel synaptic protein important for learning and memory. eLife 3, e03895-, (2014). http://www.ncbi.nlm.nih.gov/pubmed/25392983

Bhuvanendran, S. et al. Superresolution imaging of human cytomegalovirus vMIA localization in sub-mitochondrial compartments. Viruses 6, 1612-1636, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24721787

Bianchini, P., Cardarelli, F., Luca, M. D., Diaspro, A. & Bizzarri, R. Nanoscale Protein Diffusion by STED-Based Pair Correlation Analysis. PloS one 9, e99619, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24967681

Collin, L. et al. Neuronal uptake of tau/pS422 antibody and reduced progression of tau pathology in a mouse model of Alzheimer's disease. Brain : a journal of neurology 137, 2834-2846, (2014). http://www.ncbi.nlm.nih.gov/pubmed/25085375

Gallo, L. I. et al. TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells. Mol. Biol. Cell 25, 3779-3797, (2014). http://www.ncbi.nlm.nih.gov/pubmed/25232007

Guzman, C. et al. The efficacy of Raf-recruitment to H-ras depends on H-ras membrane conformer specific nanoclustering. The Journal of biological chemistry, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24569991

Herzig, B. et al. Ballchen is required for self-renewal of germline stem cells in Drosophila melanogaster. Biology open 3, 510-521, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24876388

Jepson, J. E. et al. Regulation of synaptic development and function by the Drosophila PDZ protein Dyschronic. Development 141, 4548-4557, (2014). http://www.ncbi.nlm.nih.gov/pubmed/25359729

Kannan, R., Kuzina, I., Wincovitch, S., Nowotarski, S. H. & Giniger, E. The Abl/enabled signaling pathway regulates Golgi architecture in Drosophila photoreceptor neurons. Mol. Biol. Cell 25, 2993-3005, (2014). http://www.ncbi.nlm.nih.gov/pubmed/25103244

Koch, N., Kobler, O., Thomas, U., Qualmann, B. & Kessels, M. M. Terminal axonal arborization and synaptic bouton formation critically rely on abp1 and the arp2/3 complex. PloS one 9, e97692, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24841972

Lavieu, G. et al. The Golgi ribbon structure facilitates anterograde transport of large cargoes. Mol. Biol. Cell 25, 3028-3036, (2014). http://www.ncbi.nlm.nih.gov/pubmed/25103235

Lazaro, D. F. et al. Systematic Comparison of the Effects of Alpha-synuclein Mutations on Its Oligomerization and Aggregation. PLoS Genet 10, e1004741, (2014). http://www.ncbi.nlm.nih.gov/pubmed/25393002

Lemenager, G., De Luca, E., Sun, Y. P. & Pompa, P. P. Super-resolution fluorescence imaging of biocompatible carbon dots. Nanoscale 6, 8617-8623, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24983856

Mace, E. M. & Orange, J. S. Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy. Journal of Visualized Experiments, e51100, (2014). http://www.jove.com/video/51100

Mace, E. M. & Orange, J. S. Lytic immune synapse function requires filamentous actin deconstruction by Coronin 1A. Proceedings of the National Academy of Sciences of the United States of America 111, 6708-6713, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24760828

Manzo, C. et al. PSF decomposition of nanoscopy images via Bayesian analysis unravels distinct molecular organization of the cell membrane. Scientific reports 4, 4354, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24619088

Matsukawa, H. et al. Netrin-G/NGL Complexes Encode Functional Synaptic Diversification. J. Neurosci. 34, 15779-15792, (2014). http://www.jneurosci.org/cgi/content/abstract/34/47/15779

Nogueira, C. et al. SLY1 and Syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum. eLife 3, (2014). http://elifesciences.org/elife/3/e02784.full.pdf

Park, S. W. et al. Intracellular amyloid beta alters the tight junction of retinal pigment epithelium in 5XFAD mice. Neurobiol. Aging 35, 2013-2020, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24709310

Prakasam, H. S. et al. A1 adenosine receptor-stimulated exocytosis in bladder umbrella cells requires phosphorylation of ADAM17 Ser-811 and EGF receptor transactivation. Mol. Biol. Cell 25, 3798-3812, (2014). http://www.ncbi.nlm.nih.gov/pubmed/25232008

Revelo, N. H. et al. A new probe for super-resolution imaging of membranes elucidates trafficking pathways. The Journal of cell biology 205, 591-606, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24862576

Saka, S. K. et al. Correlated optical and isotopic nanoscopy. Nature communications 5, 3664, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24718107

Sato, Y. et al. MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nature communications 5, 5266, (2014). http://www.ncbi.nlm.nih.gov/pubmed/25366663

Satoh, D. et al. aPKClambda maintains the integrity of the glomerular slit diaphragm through trafficking of nephrin to the cell surface. J. Biochem. 156, 115-128, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24700503

Westin, L., Reuss, M., Lindskog, M., Aperia, A. & Brismar, H. Nanoscopic spine localization of Norbin, an mGluR5 accessory protein. BMC neuroscience 15, 45, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24670218

Wijetunge, L. S., Angibaud, J., Frick, A., Kind, P. C. & Nagerl, U. V. Stimulated emission depletion (STED) microscopy reveals nanoscale defects in the developmental trajectory of dendritic spine morphogenesis in a mouse model of fragile X syndrome. The Journal of neuroscience : the official journal of the Society for Neuroscience 34, 6405-6412, (2014). http://www.ncbi.nlm.nih.gov/pubmed/24790210

2013

Catrin, R. et al. Using STED and ELSM confocal microscopy for a better knowledge of fused silica polished glass interface. Optics express 21, 29769-29779, (2013). http://www.ncbi.nlm.nih.gov/pubmed/24514527

Fukata, Y. et al. Local palmitoylation cycles define activity-regulated postsynaptic subdomains. The Journal of cell biology 202, 145-161, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23836932

Helm, C., Schemel, S. & Bleidorn, C. Temporal plasticity in annelid development--ontogeny of Phyllodoce groenlandica (Phyllodocidae, Annelida) reveals heterochronous patterns. Journal of experimental zoology. Part B, Molecular and developmental evolution 320, 166-178, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23440901

Helm, C., Weigert, A., Mayer, G. & Bleidorn, C. Myoanatomy of Myzostoma cirriferum (Annelida, Myzostomida): implications for the evolution of the myzostomid body plan. J. Morphol. 274, 456-466, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23280634

Mayer, G., Kauschke, S., Rudiger, J. & Stevenson, P. A. Neural markers reveal a one-segmented head in tardigrades (water bears). PloS one 8, e59090, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23516602

Oku, S., Takahashi, N., Fukata, Y. & Fukata, M. In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes. The Journal of biological chemistry 288, 19816-19829, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23687301

Reimold, C., Defeu Soufo, H. J., Dempwolff, F. & Graumann, P. L. Motion of variable-length MreB filaments at the bacterial cell membrane influences cell morphology. Mol. Biol. Cell 24, 2340-2349, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23783036

Schvarzstein, M., Pattabiraman, D., Bembenek, J. N. & Villeneuve, A. M. Meiotic HORMA domain proteins prevent untimely centriole disengagement during Caenorhabditis elegans spermatocyte meiosis. Proceedings of the National Academy of Sciences of the United States of America 110, E898-907, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23401519

Sobek, S. et al. Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I. Nucleic Acids Res. 41, 9848-9857, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23982517

Wang, I. H. et al. Tracking viral genomes in host cells at single-molecule resolution. Cell host & microbe 14, 468-480, (2013). http://www.ncbi.nlm.nih.gov/pubmed/24139403

Wilfling, F. et al. Triacylglycerol synthesis enzymes mediate lipid droplet growth by relocalizing from the ER to lipid droplets. Dev. Cell 24, 384-399, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23415954

Zanella, R. et al. Towards real-time image deconvolution: application to confocal and STED microscopy. Scientific reports 3, 2523, (2013). http://www.ncbi.nlm.nih.gov/pubmed/23982127

2012

Baer, A. & Mayer, G. Comparative anatomy of slime glands in onychophora (velvet worms). J. Morphol. 273, 1079-1088, (2012). http://dx.doi.org/10.1002/jmor.20044

Bianchini, P. & Diaspro, A. Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam. Journal of microscopy 245, 225-228, (2012). http://www.ncbi.nlm.nih.gov/pubmed/22171566

Bianchini, P., Harke, B., Galiani, S., Vicidomini, G. & Diaspro, A. Single-wavelength two-photon excitation-stimulated emission depletion (SW2PE-STED) superresolution imaging. Proceedings of the National Academy of Sciences of the United States of America 109, 6390-6393, (2012). http://www.ncbi.nlm.nih.gov/pubmed/22493221

Coltharp, C. & Xiao, J. Superresolution microscopy for microbiology. Cellular microbiology 14, 1808-1818, (2012). http://www.ncbi.nlm.nih.gov/pubmed/22947061

Giese, A. P. et al. Gipc1 has a dual role in Vangl2 trafficking and hair bundle integrity in the inner ear. Development 139, 3775-3785, (2012). http://www.ncbi.nlm.nih.gov/pubmed/22991442

Karlsson, T., Turkina, M. V., Yakymenko, O., Magnusson, K. E. & Vikstrom, E. The Pseudomonas aeruginosa N-acylhomoserine lactone quorum sensing molecules target IQGAP1 and modulate epithelial cell migration. PLoS pathogens 8, e1002953, (2012). http://www.ncbi.nlm.nih.gov/pubmed/23071436

Kinoshita, Y., Kalir, T., Dottino, P. & Kohtz, D. S. Nuclear distributions of NUP62 and NUP214 suggest architectural diversity and spatial patterning among nuclear pore complexes. PloS one 7, e36137, (2012). http://www.ncbi.nlm.nih.gov/pubmed/22558357

Lv, C., Gould, T. J., Bewersdorf, J. & Zenisek, D. High-resolution optical imaging of zebrafish larval ribbon synapse protein RIBEYE, RIM2, and CaV 1.4 by stimulation emission depletion microscopy. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 18, 745-752, (2012). http://www.ncbi.nlm.nih.gov/pubmed/22832038

Mace, E. M. & Orange, J. S. Dual channel STED nanoscopy of lytic granules on actin filaments in natural killer cells. Communicative & integrative biology 5, 184-186, (2012). http://www.ncbi.nlm.nih.gov/pubmed/22808328

Mitchell-Jordan, S. et al. Features of endogenous cardiomyocyte chromatin revealed by super-resolution STED microscopy. Journal of molecular and cellular cardiology 53, 552-558, (2012). http://www.ncbi.nlm.nih.gov/pubmed/22846883

Opazo, F. et al. Aptamers as potential tools for super-resolution microscopy. Nature methods 9, 938-939, (2012). http://www.ncbi.nlm.nih.gov/pubmed/23018995

2011

Bianchini, P., Harke, B., Galiani, S. & Diaspro, A. Two-photon excitation STED-CW microscopy. Proc. SPIE 7903, 79032V, (2011). http://dx.doi.org/10.1117/12.879790

Diaspro, A. et al. Two-Photon Excitation for Optical Nanoscopy and Light-Sheet Illumination Microscopy. . Microscopy and Analysis 25, (2011). http://www.microscopy-analysis.com/magazine/issues/two-photon-excitation-optical-nanoscopy-and-light-sheet-illumination-microscopy

Gugel, H., Giske, A., Dyba, M. & Sieber, J. Confocal imaging at the nanoscale with two-color STED microscopy.  7905, 79050X-79050X-79057, (2011). http://dx.doi.org/10.1117/12.874779

Heslop-Harrison, J. S. & Schwarzacher, T. Organisation of the plant genome in chromosomes. Plant J. 66, 18-33, (2011). http://www.ncbi.nlm.nih.gov/pubmed/21443620

Osinska, M. et al. Distinct expression, localization and function of two Rab7 proteins encoded by paralogous genes in a free-living model eukaryote. Acta biochimica Polonica 58, 597-607, (2011). http://www.ncbi.nlm.nih.gov/pubmed/22030555

Overlack, N. et al. Direct interaction of the Usher syndrome 1G protein SANS and myomegalin in the retina. Biochimica et biophysica acta 1813, 1883-1892, (2011). http://www.ncbi.nlm.nih.gov/pubmed/21767579

Pastorino, L. et al. Permeability Variation Study in Collagen-Based Polymeric Capsules. BioNanoSci. 1, 192-197, (2011). http://dx.doi.org/10.1007/s12668-011-0020-7

Pellett, P. A. et al. Two-color STED microscopy in living cells. Biomedical optics express 2, 2364-2371, (2011). http://www.ncbi.nlm.nih.gov/pubmed/21833373

Perkel, J. M. A STED-y route to commercialization. BioTechniques 50, 357-363, (2011). http://www.ncbi.nlm.nih.gov/pubmed/21781034

Rak, G. D., Mace, E. M., Banerjee, P. P., Svitkina, T. & Orange, J. S. Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse. PLoS Biol. 9, e1001151, (2011). http://www.ncbi.nlm.nih.gov/pubmed/21931536

2007-2010

Jablonka, S., Beck, M., Lechner, B. D., Mayer, C. & Sendtner, M. Defective Ca2+ channel clustering in axon terminals disturbs excitability in motoneurons in spinal muscular atrophy. The Journal of cell biology 179, 139-149, (2007). http://www.ncbi.nlm.nih.gov/pubmed/17923533

Geumann, U., Barysch, S. V., Hoopmann, P., Jahn, R. & Rizzoli, S. O. SNARE function is not involved in early endosome docking. Mol. Biol. Cell 19, 5327-5337, (2008). http://www.ncbi.nlm.nih.gov/pubmed/18843044

Barysch, S. V., Aggarwal, S., Jahn, R. & Rizzoli, S. O. Sorting in early endosomes reveals connections to docking- and fusion-associated factors. Proceedings of the National Academy of Sciences of the United States of America 106, 9697-9702, (2009). http://www.ncbi.nlm.nih.gov/pubmed/19487677

Cseresnyes, Z., Schwarz, U. & Green, C. M. Analysis of replication factories in human cells by super-resolution light microscopy. BMC cell biology 10, 88, (2009). http://www.ncbi.nlm.nih.gov/pubmed/20015367

Fitzpatrick, J. A. et al. STED nanoscopy in living cells using Fluorogen Activating Proteins. Bioconjugate chemistry 20, 1843-1847, (2009). http://www.ncbi.nlm.nih.gov/pubmed/20976031

Fouquet, W. et al. Maturation of active zone assembly by Drosophila Bruchpilot. The Journal of cell biology 186, 129-145, (2009). http://www.ncbi.nlm.nih.gov/pubmed/19596851

Meyer, A. C. et al. Tuning of synapse number, structure and function in the cochlea. Nature neuroscience 12, 444-453, (2009). http://www.ncbi.nlm.nih.gov/pubmed/19270686

Prokopi, M. et al. Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures. Blood 114, 723-732, (2009). http://www.ncbi.nlm.nih.gov/pubmed/19369228

Schroder, J., Benink, H., Dyba, M. & Los, G. V. In vivo labeling method using a genetic construct for nanoscale resolution microscopy. Biophysical journal 96, L01-03, (2009). http://www.ncbi.nlm.nih.gov/pubmed/19134467

Coffinier, C. et al. Direct synthesis of lamin A, bypassing prelamin a processing, causes misshapen nuclei in fibroblasts but no detectable pathology in mice. The Journal of biological chemistry 285, 20818-20826, (2010). http://www.ncbi.nlm.nih.gov/pubmed/20439468

Farahani, J. N., Schibler, M. J. & Bentolila, L. A. Stimulated Emission Depletion (STED) Microscopy: from Theory to Practice Microscopy: Science, Technology, Applications and Education, 1539-1547, (2010). http://www.formatex.info/microscopy4/1539-1547.pdf

Felts, R. L. et al. 3D visualization of HIV transfer at the virological synapse between dendritic cells and T cells. Proceedings of the National Academy of Sciences of the United States of America 107, 13336-13341, (2010). http://www.ncbi.nlm.nih.gov/pubmed/20624966

Geis, C. et al. Stiff person syndrome-associated autoantibodies to amphiphysin mediate reduced GABAergic inhibition. Brain : a journal of neurology 133, 3166-3180, (2010). http://www.ncbi.nlm.nih.gov/pubmed/20884644

Hoopmann, P. et al. Endosomal sorting of readily releasable synaptic vesicles. Proceedings of the National Academy of Sciences of the United States of America 107, 19055-19060, (2010). http://www.ncbi.nlm.nih.gov/pubmed/20956291

Kamin, D. et al. High- and low-mobility stages in the synaptic vesicle cycle. Biophysical journal 99, 675-684, (2010). http://www.ncbi.nlm.nih.gov/pubmed/20643088

Leone, S., Cornetta, T., Basso, E. & Cozzi, R. Resveratrol induces DNA double-strand breaks through human topoisomerase II interaction. Cancer Lett. 295, 167-172, (2010). http://www.sciencedirect.com/science/article/pii/S0304383510001321

Morozova, K. S. et al. Far-red fluorescent protein excitable with red lasers for flow cytometry and superresolution STED nanoscopy. Biophysical journal 99, L13-15, (2010). http://www.ncbi.nlm.nih.gov/pubmed/20643047

Opazo, F. et al. Limited intermixing of synaptic vesicle components upon vesicle recycling. Traffic 11, 800-812, (2010). http://www.ncbi.nlm.nih.gov/pubmed/20230528

Opazo, F. & Rizzoli, S. O. The fate of synaptic vesicle components upon fusion. Communicative & integrative biology 3, 427-429, (2010). http://www.ncbi.nlm.nih.gov/pubmed/21057631

Petralia, R. S. et al. Organization of NMDA receptors at extrasynaptic locations. Neuroscience 167, 68-87, (2010). http://www.ncbi.nlm.nih.gov/pubmed/20096331

Rickman, C. et al. t-SNARE protein conformations patterned by the lipid microenvironment. The Journal of biological chemistry 285, 13535-13541, (2010). http://www.ncbi.nlm.nih.gov/pubmed/20093362

Sieber, J. J. & Schué, A. STED-Mikroskopie. Optik & Photonik 5, 36-39, (2010). http://dx.doi.org/10.1002/opph.201190077