When imaging challenging specimens, unwanted light due to reflection or auto fluorescence can be a problem. This reflected light is detected alongside your sample's fluorescence emissions, interfering with the accuracy of your images. LightGate is a filter-free method to remove unwanted signal from the image data. Leica's LightGate is an adjustable temporal window that removes these unwanted emissions to ensure that only fluorescence signals are collected for imaging. The White Light Laser's pulsed output and the HyD's quantifiable signal detection make this advanced imaging technique possible.
Primary culture of rat cortical neurons, excitation and detection settings were adjusted to optimal image contrast
|Nestin (neuronal stem cells)||cy2||495||504-545|
|DCX (immature neurons)||cy3||560||559-624|