Applications Leica EM HPM100
Freeze-Fracture Replication of Pyramidal Cells
12 September 2011
Myelinated axons
by Dr. Akos Kulik, Department of Anatomy and Cell Biology, University of Freiburg, Germany
Protocol
Frozen samples (90 µm thick slices frozen by Leica EM HPM100) were inserted into a double replica table and then fractured into two pieces at -130°C (after insertion of the tissue into the Leica EM BAF060 the samples should be left in the chamber for 20 min to reath the -130°C)...
Mouse cerebellum
12 September 2011
Cerebellar slice of mouse brain. High-pressure frozen in a Leica EM HPM 100 with ethanol as synchronization fluid.
Courtesy of: Dr. sc. nat. Andres Kaech, Center for Microscopy and Image Analysis, University of Zurich, Switzerland
(by Dr. sc. nat. Andres Kaech, Center for Microscopy and Image Analysis, University of Zurich, Switzerland)
This Application Note describes the preparation method
"Mouse cerebellum"
using the Leica EM HPM100.
Protocol:
Cerebellar slices were cultured on Millicell filter membranes. Discs of 4 mm were cut a a 4 mm biopsy punch on a teflon surface. The filder discs containing the brain slices were transferred with a tweezers into the 100 micrometer cavity of a 6 mm aluminium specimen carrier ...
Hep-2 cells infected with Chlamydia pneumonia
12 September 2011
Hep-2 cells infected with Chlamydia pneumoniae
Courtesy of: Dr. sc. nat. Andres Kaech, Center for Microscopy and Image Analysis, University of Zürich, Switzerland)
(by Dr. sc. nat. Andres Kaech, Center for Microscopy and Image Analysis, University of Zürich, Switzerland)
This Application Note describes the preparation method
"Hep-2 cells infected with Chlamydia pneumoniae"
using the Leica EM HPM100.
Protocol:
Hep-2 cells infected with Chlamydia pneumonia grown on carbon coated 6 mm Sapphire disc. High-pressure frozen in a Leica EM HPM100 with ethanol as synchronization fluid ...
CEMOVIS of Yeast
06 April 2011
Courtesy of Dr. Jonathan O’Driscoll, Dr. Daniel Kofi Clare and Prof. Helen Saibil,Department of Cryostallography, Birkbeck, University of London
Protocol:
The sections are of yeast frozen with a Leica EM HPM100 in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer so that a final MES concentration of 50mM and a dextran concentration of 20% was achieved .....
(by Dr. Jonathan O’Driscoll, Dr. Daniel Kofi Clare and Prof. Helen Saibil, prepared at the Department of Cryostallography, Birkbeck, University of London)
This Application Note describes the preparation method
"CEMOVIS of Yeast"
using Leica EM HPM100, Leica EM UC7 , Leica EM FC7
Click here to download the Application Note
Visit Birkbeck, University of London, Department of Biological Sciences
Superior Cryopreparation of Biological and Industrial Samples - Commercially available hand creams
21 July 2010
Kim Rensing Ph.D. at the University of British Columbia, BioImaging Facility, Vancouver, Canada
Protocol:
Hand creams having different water contents were applied into the 100 µm cavities of two 3 mm type A sample carriers which were then closed cream sides inwards. ......
(Samples prepared by Kim Rensing Ph.D. at the University of British Columbia, BioImaging Facility, Vancouver, Canada)
This Application Note describes the preparation method
"Superior Cryopreparation of Biological and Industrial Samples - Commercially available hand creams"
using the Leica EM HPM100 and Leica EM VCT100 and Leica EM BAF060