Applications Leica EM BAF060
Freeze-Fracture Replication of Pyramidal Cells
12 September 2011
Myelinated axons
by Dr. Akos Kulik, Department of Anatomy and Cell Biology, University of Freiburg, Germany
Protocol
Frozen samples (90 µm thick slices frozen by Leica EM HPM100) were inserted into a double replica table and then fractured into two pieces at -130°C (after insertion of the tissue into the Leica EM BAF060 the samples should be left in the chamber for 20 min to reath the -130°C)...
Superior Cryopreparation of Biological and Industrial Samples - Commercially available hand creams
21 July 2010
Kim Rensing Ph.D. at the University of British Columbia, BioImaging Facility, Vancouver, Canada
Protocol:
Hand creams having different water contents were applied into the 100 µm cavities of two 3 mm type A sample carriers which were then closed cream sides inwards. ......
(Samples prepared by Kim Rensing Ph.D. at the University of British Columbia, BioImaging Facility, Vancouver, Canada)
This Application Note describes the preparation method
"Superior Cryopreparation of Biological and Industrial Samples - Commercially available hand creams"
using the Leica EM HPM100 and Leica EM VCT100 and Leica EM BAF060