Atomic Force Microscopy (AFM) Integration of Veeco BioScope™ II with Leica DMI6000 B
It is a technology and marketing partnership.
The initial phase of the collaboration focuses on the innovation and integration of Veeco's BioScope II Atomic Force Microscopy (AFM) with Leica's DMI series of inverted light microscopes with the benefit of imaging, probing & manipulating biological systems: from the living cell down to single molecules. In this way both companies support high resolution imaging for biological and nanomedical research applications to investigate ways to impact human health.
The BioScope II is well designed to fit into the optical path of the Leica DMI inverted microscope without compromising optical functions. The open, ergonomic design of the BioScope II allows all transmitted light contrasting, fluorescence illumination and combined techniques. The BioScope II XY-scanning sample stage provides a dynamic range of scan sizes.
To permit imaging of taller biological structures, such as cells, the BioScope II offers a Z range greater than 15µm. Meanwhile, an X-Y range that exceeds 150µm lets users better match cell/sample size to scan area and easily correlate AFM data with optical/fluorescence images.
Application flexibility
- in situ high resolution imaging and analysis of molecules and cells
- Force measurements / spectroscopy protein-protein and ligand-receptor (un)binding, molecular (un)folding, nanomanipulations, adhesion and elasticity of cells
User benefits
- Consistent sharp images or overlays
- Resolution from µm to nm scale
- Using advanced optical techniques including DIC and fluorescence in combination with AFM
- Studying biological samples in real-time and under physiological conditions
- Unique access to the sample / tip
- Versatile and easy to use
- Supporting a variety of imaging modes, e.g. contact and tapping mode including phase imaging
Integration of Veeco BioScope II with Leica DMI6000 B
Overlay triple fluorescence labeled cells with AFM image.HeLa cells by courtesy of Dr. Frank Lafont, Pasteur Institute Lille
Integration of Veeco BioScope II with Leica DMI6000 B
AFM height image (100 x 100 µm square). HeLa cells by courtesy of Dr. Frank Lafont, Pasteur Institute Lille