Brain research: Single neuron excision for RNA analysis - Improve your workflow with laser microdissection - Parkinson’s disease is a common progressive neurodegenerative disorder. It is connected with cell death of dopamine-releasing neurons in the substantia nigra. Differences in gene expression patterns between individual dopamine-releasing neurons of disease affected and healthy individuals allow defining target genes for therapies. For RNA analysis, single cell resolution is crucial, as investigating the whole tissue is meaningless. Analyzing mixtures of dopamine-releasing neurons and all other brain cells distorts the result.
Cancer research: Extract undiluted cancer tissue for DNA mutation analysis - Improve your workflow with Laser Microdissection - DNA mutations lead to abnormal proteins or missing functional proteins, which can cause cells to multiply uncontrollably and become cancerous. To find and understand the underlying mutation for a specific cancer type, extraction of pure tumor material is extremely important. This is challenging especially if only a limited amount of cancerous tissue is available (small metastasis, for example).
Brain Research: Collect pure starting material for proteomics - Improve your workflow with Laser Microdissection - Many brain diseases result from protein malfunction, misfolding and agglutination. For this reason protein analysis is the key to understanding causes of, and discovering therapies for, many cerebral defects.
During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na Agricultura/USP (CENA), the power of laser microdissection using the Leica LMD systems was demonstrated. One special focus was on plant dissection which needs a high laser power.
Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a perfect tool for this purpose and how this was taught in the course of a workshop held in Brazil. With the Leica LMD system pure tumor material can be selected and dissected for downstream analysis to ensure 100% pure starting material without any risk of cross contamination with healthy cells.
The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as it allows to separate distinct brain layers or even to isolate individual neurons.
During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome. Another topic was the dissection of individual chromosomes from chromosome spreads. Leica specialists held a short training session with the LMD. After this, new LMD users were able to run the system and practiced how to dissect chromosomes and collect single chromosomes for downstream analysis.
Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or sequencing techniques. Here we describe the successful combination of the Leica Microsystems LMD system and Qiagen kits for purification of nucleic acids even from little amounts.
Secondary plant metabolites are involved in various plant physiological and biochemical processes, allowing plants to interact successfully with their biotic and abiotic environment. Distribution patterns of these compounds at the tissue and cellular level show how plant defense systems are organized to manage environmental challenges in a targeted and precise way.
A characteristic sign of M. Parkinson is the deterioration of dopaminergic neurons in the mid-brain, specifically in the substantia nigra (SN, black substance). Different causes and forms of this disease have been identified. In the case of the genetic familial form, for example, it has been possible to identify various genes that have a causal influence for M. Parkinson.
Arbuscular mycorrhizal (AM) fungi are an essential feature of the biology and ecology of most terrestrial plants and, as biofertilizers, AM fungi are an emerging issue in many projects focused on low-input agriculture practices. The identification of the events that lead to the formation of an AM, including the mechanisms involved in nutrient transfer, will be a challenging objective for a better exploitation of AMs in agricultural programs.
The comprehension of stem cell biology and its molecular basis is now acquiring paramount importance in cancer research. The need to look at a single, possibly living, cell makes fluorescence microscopy and confocal microscopy invaluable allies in the study of stem cells.
Different areas of the Central Nervous System (CNS) display a specific and selective gene expression profile. Here, we used the Laser Microdissection system Leica LMD6500 to study region-specific mRNA expression in the adult mouse retina and hippocampus.
Due to its instability, RNA is generally more difficult to work with than DNA. RNA does not have the stability of the DNA double helix. To obtain the best results when dealing with RNA it is essential to start out with high-quality material and to carry out particularly careful quality control before and after processing. The so-called RIN (RNA Integrity Number) is an indicator of the quality of RNA. On a scale of 1–10, a RIN value of 1 indicates that the RNA is completely degraded and a RIN value of 10 that the RNA is fully intact. The higher the RIN number, the better the RNA quality. Wherever possible, material with high RIN numbers should always be used.
Laser dissection is used in a large number of research fields, e.g. neurology, cancer research, plant analysis. Here, user report on the research results they have attained by using laser microdissection.
New and far-reaching applications have recently been opened up in the field of laser microdissection. In addition to conventional dissection, the laser microdissection system (LMD) of Leica Microsystems is an excellent tool for marking relevant structures, offering highly specific laser manipulation of selected areas. This laser marking function is useful for applications such as CLEM, NanoSIMS as well as in the live cell sector.
Your role in pharmaceutical and chemical manufacturing, research and development requires microscope, camera and software solutions to help you clearly and precisely visualize, analyze, and document your results while ensuring the highest level of accuracy. Improve experimental efficiency and reduce strain from repetitive tasks during production while achieving the excellent quality results you need.