Contact Us

LMD6 & LMD7 Laser Microdissection Microscopes

Dissection Perfection

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Now there is single-cell Deep Visual Proteomics (scDVP), a technology that integrates high-content imaging, laser microdissection, and multiplexed mass spectrometry (MS) for tissue characterization at the single cell level.

Download the complete list of LMD publications

Single cell applications

Association between RMTg Neuropeptide Genes and Negative Effect during Alcohol Withdrawal in Mice
Fu Y, Li W, Mai Y, Guan J, Ding R, Hou J, Chen B, Cao G, Sun S, Tang Y, and Fu R
Int. J. Mol. Sci. 2024, 25(5), 2933; Doi: 10.3390/ijms25052933

Digital PCR for Single-Cell Analysis
Fang W, Liu X, Maiga M, Cao W, Mu Y, Yan Q, and Zhu Q
Biosensors 2024, 14(2), 64

DMHPpp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication
Tokizane K, Brace CS, Imai SI
Cell Metab. 2024 Jan 1:S1550-4131(23)00462-X. doi: 10.1016/j.cmet.2023.12.011.

DNA Research

Paired qualitative and quantitative analysis of bacterial microcolonies in the tonsils of patients with tonsillar hyperplasia
Teh J, Biswas K, Waldvogel-Thurlow S, Broderick D, Clark ST, Johnston J, Mackenzie BW, Douglas R
Microbes Infect. 2024 Mar 5:105317. doi: 10.1016/j.micinf.2024.105317.

Morphologic and Molecular Heterogeneity of High-grade Serous Carcinoma Precursor Lesions
Chien Y-W, Wang Y, Huang P, Lawson BC, Kolin DL, Chui MH, Vang R, Numan TA, Soong TR, Wang BG, Smith SA, Chen C-L, Stone R, Douville C, Wang T-L, Shih lM
The American Journal of Surgical Pathology ():10.1097/PAS.0000000000002187, February 1, 2024.

Genome-wide analysis of DNA methylation in pseudomyxoma peritonei originated from appendiceal neoplasms
Takane K, Cai T, Noguchi R, Gohda Y, Ikenoue T, Yamaguchi K, Ota Y, Kiyomatsu T, Yano H, Fukuyo M, Seki M, Bahityar R, Kaneda A, Furukawa Y
Oncology. 2024 Jan 23. doi: 10.1159/000536219.

RNA Research

Deletion of Cardiac Fgf23 Impairs Myocardial Energy Metabolism and Ameliorates Fibrosis in a Pressure Overload Model in Mice
Erben R, Latic N, Sun N, Buck A, Zupcic A, Hofer M, Chang W, Walch A, Grabner A
Research Square, Dec 22nd 2023

Periportal hepatocyte proliferation at midgestation governs maternal glucose homeostasis in mice
Kozuki S, Kabata M, Sakurai S, Iwaisako K, Nishimura T, Toi M, Yamamoto T, Toyoshima F
Commun Biol. 2023 Dec 4;6(1):1226. doi: 10.1038/s42003-023-05614-3.

Transcriptome profile of subsynaptic myonuclei at the neuromuscular junction in embryogenesis
Ohkawara B, Kurokawa M, Kanai A, Imamura K, Chen G, Zhang R, Masuda A, Higashi K, Mori H, Suzuki Y, Kurokawa K, Ohno K
J Neurochem. 2023 Nov 23. doi: 10.1111/jnc.16013.

Highlights 2023: SPARCS (spatially resolved CRISPR screening)
Schmacke et al.
https://doi.org/10.1101/2023.06.01.542416

Proteomics, Lipidomics, Metabolomics

MALDI-MSI-LC-MS/MS Workflow for Single-Section Single Step Combined Proteomics and Quantitative Lipidomics
Hendriks TFE, Krestensen KK, Mohren R, Vandenbosch M, De Vleeschouwer S, Heeren RMA, and Cuypers E
Anal. Chem. 2024, DOI: 10.1021/acs.analchem.3c05850, March 4, 2024

Concentric Hybrid Nanoelectrospray Ionization-Atmospheric Pressure Chemical Ionization Source for High-Coverage Mass Spectrometry Analysis of Single-Cell Metabolomics
Xu T, Li H, Dou P, Luo Y, Pu S, Mu H, Zhang Z, Feng D, Hu X, Wang T, Tan G, Chen C, Li H, Shi X, Hu C, Xu G
Adv Sci (Weinh). 2024 Feb 15:e2306659. doi: 10.1002/advs.202306659.

Differential Glycoform Analysis of MUC1 Derived from Biological Specimens Using an Antibody-Overlay Lectin Microarray
Matsuda A, Boottanun P, Koizumi S, Nagai M, Kuno A
Methods Mol Biol. 2024;2763:223-236. doi: 10.1007/978-1-0716-3670-1_19.

Highlight 2022: Deep Visual Proteomics (DVP)
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Fluorescence Applications

Association between RMTg Neuropeptide Genes and Negative Effect during Alcohol Withdrawal in Mice
Fu Y, Li W, Mai Y, Guan J, Ding R, Hou J, Chen B, Cao G, Sun S, Tang Y, and Fu R
Int. J. Mol. Sci. 2024, 25(5), 2933; Doi: 10.3390/ijms25052933

DMHPpp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication
Tokizane K, Brace CS, Imai SI
Cell Metab. 2024 Jan 1:S1550-4131(23)00462-X. doi: 10.1016/j.cmet.2023.12.011.

An automated and fast sample preparation workflow for laser microdissection guided ultrasensitive proteomics
Makhmut A, Qin D, Hartlmayr D, Seth A, and Coscia F
bioRxiv, Dec 1st 2023

Cancer Research

Concentric Hybrid Nanoelectrospray Ionization-Atmospheric Pressure Chemical Ionization Source for High-Coverage Mass Spectrometry Analysis of Single-Cell Metabolomics
Xu T, Li H, Dou P, Luo Y, Pu S, Mu H, Zhang Z, Feng D, Hu X, Wang T, Tan G, Chen C, Li H, Shi X, Hu C, Xu G
Adv Sci (Weinh). 2024 Feb 15:e2306659. doi: 10.1002/advs.202306659.

Microenvironment Shapes Cell State, Plasticity, and Heterogeneity of Small Cell Lung Cancer
Desai P, Takahashi N, Kumar R, Nichols S, Malin J, Hunt A, Schultz C, Cao Y, Tillo D, Nousome D, Chauhan L, Sciuto L, Jordan K, Rajapakse V, Lissa D, Zhang Y, Kumar S, Pongor L, Singh A, Schroder B, Kumar Sharma A, Chang T, Vilimas R, Pinkiert D, Graham C, Butcher D, Warner A, Sebastian R, Mahon M, Baker K, Cheng J, Berger A, Lake R, Abel M, Krishnamurthy M, Chrisafis G, Fitzgerald P, Micheal Nirula M, Goyal S, Atkinson D, Bateman NW, Abulez T, Nair G, Apolo A, Guha U, Karim B, El Meskini R, Ohler ZW, Kumar Jolly M, Schaffer A, Ruppin E, Kleiner D, Miettinen M, Brown GT, Hewitt S, Conrads T, Thomas A
bioRxiv, Feb 14., 2024

Morphologic and Molecular Heterogeneity of High-grade Serous Carcinoma Precursor Lesions
Chien Y-W, Wang Y, Huang P, Lawson BC, Kolin DL, Chui MH, Vang R, Numan TA, Soong TR, Wang BG, Smith SA, Chen C-L, Stone R, Douville C, Wang T-L, Shih lM
The American Journal of Surgical Pathology ():10.1097/PAS.0000000000002187, February 1, 2024.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Neurosciences

Association between RMTg Neuropeptide Genes and Negative Effect during Alcohol Withdrawal in Mice
Fu Y, Li W, Mai Y, Guan J, Ding R, Hou J, Chen B, Cao G, Sun S, Tang Y, and Fu R
Int. J. Mol. Sci. 2024, 25(5), 2933; Doi: 10.3390/ijms25052933

DMHPpp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication
Tokizane K, Brace CS, Imai SI
Cell Metab. 2024 Jan 1:S1550-4131(23)00462-X. doi: 10.1016/j.cmet.2023.12.011.

Ascorbic acid and its transporter SVCT2, affect radial glia cells differentiation in postnatal stages
Saldivia N, Salazar K, Cifuentes M, Espinoza F, Harrison FE, Nualart F
Glia. 2024 Jan 5. doi: 10.1002/glia.24498.

Clinical/Pathology/Immunology Research Applications

Paired qualitative and quantitative analysis of bacterial microcolonies in the tonsils of patients with tonsillar hyperplasia
Teh J, Biswas K, Waldvogel-Thurlow S, Broderick D, Clark ST, Johnston J, Mackenzie BW, Douglas R
Microbes Infect. 2024 Mar 5:105317. doi: 10.1016/j.micinf.2024.105317.

Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury
Mao Y, Chen Y, Li Y, Ma L, Wang X, Wang Q, He A, Liu X, Dong T, Gao W, Xu Y, Liu L, Ren L, Liu Q, Zhou P, Hu B, Zhou Y, Tian R, Shi ZL
Cell Rep. 2024 Jan 18;43(2):113689. doi: 10.1016/j.celrep.2024.113689.

Thymic stromal lymphopoietin induces IL-4/IL-13 from T cells to promote sebum secretion and adipose loss
Choa R, Harris JC, Yang E, Yokoyama Y, Okumura M, Kim M, To J, Lou M, Nelson A, Kambayashi T
J Allergy Clin Immunol. 2023 Dec 27:S0091-6749(23)02543-5. doi: 10.1016/j.jaci.2023.11.923.

Developmental Research, Epigenetics  & Aging

PIBF1 regulates trophoblast syncytialization and promotes cardiovascular development
Lee JG, Yon JM, Kim G, Lee SG, Kim CY, Cheong SA, Kim HY, Yu J, Kim K, Sung YH, Yoo HJ, Woo DC, Rho JK, Ha CH, Pack CG, Oh SH, Lim JS, Han YM, Hong EJ, Seong JK, Lee HW, Lee SW, Lee KU, Kim CJ, Nam SY, Cho YS, Baek IJ
Nat Commun. 2024 Feb 19;15(1):1487. doi: 10.1038/s41467-024-45647-8.

Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling
Costes C, Navarro Sanz S, Calatayud C, Soriano A, Mameri H, Terrier N, Francin-Allami M
BMC Genom Data. 2024 Feb 7;25(1):14. doi: 10.1186/s12863-024-01198-x.

Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction
Gao M, Zhang T, Chen T, Chen Z, Zhu Z, Wen Y, Qin S, Bao Y, Zhao T, Li H, Liu L, Hao M, Wang J, Wang F, Wang H, Zhou B, Zhang H, Xia G, Wang C
Theranostics. 2024 Jan 27;14(4):1371-1389. doi: 10.7150/thno.89878. eCollection 2024.

Plant Research

Transcriptomic analyses to summarize gene expression patterns that occur during leaf initiation of Chinese cabbage
Sun XX, Liu Z, Liu R, Bucher J, Zhao J, Visser RGF, Bonnema G
Horticulture Research, 2024

Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling
Costes C, Navarro Sanz S, Calatayud C, Soriano A, Mameri H, Terrier N, Francin-Allami M
BMC Genom Data. 2024 Feb 7;25(1):14. doi: 10.1186/s12863-024-01198-x.

Cell-type proteomic and metabolomic resolution of early and late grain filling stages of wheat endosperm
Zhang S, Ghatak A, Mohammad Bazargani M, Kramml H, Zang F, Gao S, Ramšak Ž, Gruden K, Varshney RK, Jiang D, Chaturvedi P, Weckwerth W
Plant Biotechnol J. 2023 Dec 4. doi: 10.1111/pbi.14203.

Forensics

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Protective effect of phenylpropionamides in the seed of Cannabis Sativa L. on Parkinson's disease through autophagy
Jiang YK, Li MM, Wang SY, Hao ZC, Meng X, Kuang HX, Yang BY, Liu Y
Fitoterapia. 2024 Mar 6;175:105883. doi: 10.1016/j.fitote.2024.105883.

Comprehensive Spatial Lipidomics of Formalin-Fixed Paraffin-Embedded Tissue Guided by Mass Spectrometry-Imaging
Denti V, Piazza M, Smith A, Paglia G
In: Ivanisevic, J., Giera, M. (eds) A Practical Guide to Metabolomics Applications in Health and Disease. Learning Materials in Biosciences. Springer, Cham. 

Differential Glycoform Analysis of MUC1 Derived from Biological Specimens Using an Antibody-Overlay Lectin Microarray
Matsuda A, Boottanun P, Koizumi S, Nagai M, Kuno A
Methods Mol Biol. 2024;2763:223-236. doi: 10.1007/978-1-0716-3670-1_19.

Live Cell Culture

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416

An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022

Background image
Scroll to top