Images Leica Structured Illumination

FAN rat primary culture of cortical neurons

Leica DM6000 B with Leica Structured Illumination
Objective: 100x/1.4 Oil
Description: 33 planes, 0.1µm distance
Stains: DCX-Cy3, Nestin-Cy2, DAPI

Eye anlage of E10 mouse embryo

Leica DM6000 B with Leica Structured Illumination
Objective: 100x/1.4 Oil
Description: 3D Reconstruction of 24 z-sections, 4µm z-distance, 10x/0.4
Staining: DNA: Hoechst 33342, beta-Tubulin: Alexa488, Alexa633
Sample: Courtesy of Dr. Yves Lutz, Imaging Center IGBMC, France

E10 mouse embryo

Leica DM6000 B with Leica Structured Illumination
Comparison between widefield (left) and Structured Illumination (right)
Montage of 6x4 tiles with 10x/0.4
Scan area: 4.8 x 5.3mm per embryo
Using Leica MM AF Auto Scan-slide
beta-Tubulin: Alexa633
Sample: Courtesy of Dr. Yves Lutz, Imaging Center IGBMC, France

FAN rat primary culture of cortical neurons

Leica DM6000 B with Leica Structured Illumination
Stitched mosaic of 3x2 images
Stains: DCX-Cy3
Objective: 100x/1.4 Oil

Perfect integration of the OptiGrid® module into Leica research microscopes

Mouse kidney section

63x Oil Immersion Objective

Red: Phalloidin

Green: wheat germ agglutinin

The same OptiGrid® module can be used in both upright and inverted Leica research microscopes

Mouse kidney section

Maximum projection of a stack containing 65 planes.
Green: glomeruli and convoluted tubules (wheat germ agglutinin Alexa Fluor® 488)
Blue: Nuclei (DAPI).