Applications Leica CM1850
One Pathologist’s Approach to Teaching Frozen Sectioning
19 August 2009
Anyone who has visited Dr. Peter’s website tutorial on frozen section technique knows that he is a true believer in the brush technique. Let’s learn how he teaches this valuable skill to his residents:
The first step is to find a comfortable hand position to maximize fine motor ability for this very delicate maneuver. For this, I have my students hold the brush like a pen and gently rest a finger on the anti-roll plate or at the edge of the cryostat stage.
Next, I tell them to try to write their names on the edge of the block with the brush to gain a sense of the fine movements they will use in the sectioning process.
To learn more about this pathologist’s approach to cryosectioning visit Dr. Peter’s tutorial at: http://pathologyinnovations.com/frozen_section_technique.htm.
The Art of Embedding Tissue for Frozen Section
25 May 2009
(by Stephen R. Peters, Dept. of Pathology, Hackensack University Medical Center, New Jersey, USA)
This article describes a system used for embedding of tissues for the preparation of frozen sections.
This novel system uses simple techniques and apparatus to accomplish face-down embedding in freezing-temperature steel wells.
The system is easy to learn and offers many advantages over conventional methods, including speed, high precision and predictability, and reduced tissue wastage.
The system is easily adapted in most existing cryostats, used no additional consumables, and requires minimal maintenance. (The J Histotechnol 26:11, 2003)
Paper Cryoembedding
25 May 2009
(by Stephen R. Peters et al)
The article describes a unique method of face-down tissue embedding for frozen section in freezing-temperature well bars.
Tissue is first placed face down in a precise position on a piece of lens paper. The tissue is then transferred in the exact position to an embedding well.
The block is completed while the tissue remains face down on the paper. The paper is removed during the initial trimming of the block, leaving the tissue in its original position and orientation.
The technique accomplishes precise embedding of extremely delicate and deformable tissues and arrangements of tissues.
We believe this technique will facilitate a variety of difficult embedding tasks in areas of surgical pathology, Mohs surgery, and research settings
(The J Histotechnol 26:173, 2003).