Gallery Leica LN22

The liquid nitrogen freezing device LN22 quickly converts a rotary microtome into a cryosectioning system.

The Leica LN22 attachment consists of a cryochamber, which encloses the sectioning area, and which is refrigerated by a permanent flow of liquid nitrogen, pumped from a Dewar vessel. The automatic pumping mechanism ensures a stable low temperature environment around the frozen specimen and the knife. Separate temperature control systems maintain stable temperatures of both knife and specimen.

The desired temperature setting for knife and specimen are selected on the control unit. The control unit, which continuously monitors and regulates the selected temperature parameters and controls the liquid nitrogen pumping system.
Actual temperatures of knife, specimen and cryochamber are displayed on the control unit at all times.
The cryochamber is mounted onto the microtome base plate, thus being completely isolated from the microtome feed and drive elements.