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Contrasting Methods

Contrasting methods visualize phase shifts that are otherwise invisible to the human eye and thus enable observation of unstained living samples.

Objectives that are particularly suitable for specific contrasting methods are labeled accordingly.

BD

for brightfield/incident light darkfield

PH

phase contrast objective

RC

reflection contrast objective (only with DM R)

P, POL

low strain, for quantitative polarization

/  

not for incident light, except fluorescence

LMC

modulation contrast objective (only with Leica DM IRB)