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Leica EM AFS2 Cryo Preparation

Freeze Substitution and Low Temperature Embedding System for Light and Electron Microscopy Leica EM AFS2


  • First multigene analysis of Archamoebae (Amoebozoa: Conosa) robustly reveals its phylogeny and shows that Entamoebidae represents a deep lineage of the group, Tomáš Pánek a,⇑, Eliška Zadrobílková a,b, Giselle Walker a, Matthew W. Brown c,d, Eleni Gentekaki e, Miluše Hroudová f, Seungho Kang c,d, Andrew J. Roger g,h, Alexander K. Tice c,d, Cˇestmír Vlcˇek f, Ivan Cˇ epicˇka a, a Department of Zoology, Faculty of Science, Charles University in Prague, Vinicna 7, 128 44 Prague, Czech Republic, b Centre for Epidemiology and Microbiology, National Institute of, Public Health, Srobarova 48, 100 42 Prague, Czech Republic, c Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA, d Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA, e School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand, f Department of Genomics and Bioinformatics, Institute of Molecular Genetics, Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech Republic, g Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, NS B3H 4R2, Canada, h Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada https://www.researchgate.net/profile/Matthew_Brown5/publication/291183093_First_multigene_analysis_of_Archamoebae_Amoebozoa_Conosa_robustly_reveals_its_phylogeny_and_shows_that_Entamoebidae_represents_a_deep_lineage_of_the_group/links/56bde4be08ae44da37f881e6.pdf

  • In vivo Reconstitution of Algal Triacylglycerol Production in Saccharomyces cerevisiae, Chun-Hsien Hung1, Kazue Kanehara1 and Yuki Nakamura1, 2*, 1 Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, 2 Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, Saitama, Japan, *Correspondence: Yuki Nakamura, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753380/

  • Clustering and cellular distribution characteristics of virus particles of Tomato spotted wilt virus and Tomato zonate spot virus in different plant hosts, Zhongkai Zhang1,2, Kuanyu Zheng2, Jiahong Dong2, Qi Fang2, Jian Hong3 and Xifeng Wang1*, * Correspondence: xfwang@ippcaas.cn, 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China, 2 Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223Yunnan, P.R. China. 3Center of Analysis and Measurement, Zhejiang University, Hangzhou 310058, P.R. China. http://virologyj.biomedcentral.com/articles/10.1186/s12985-016-0466-x

  • Cell Wall N-linked Mannoprotein Biosynthesis Requires Goa1p, a Putative Regulator of Mitochondrial Complex I in Candida albicans, Xiaodong She1,2, Richard Calderone1*, Michael Kruppa3, Douglas Lowman3, David Williams3, Lili Zhang2, Ying Gao2, Kasra Khamooshi1, Weida Liu2, Dongmei Li1, 1 Georgetown University Medical Center, Department of Microbiology & Immunology, Washington, DC, 20057, United States of America, 2 Institute of Dermatology, Chinese Academy of Medical Sciences (CAMS), Jiangsu Key laboratory of Molecular Biology for Skin Disease and STIs, Nanjing, 210029, China, 3 Department of Surgery, Department of Biomedical Sciences and Center of Excellence in Inflammation, Infectious Diseases, and Immunity, East Tennessee State University, Johnson City, Tennessee, 37614,United States of America, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0147175
  • Nanoscale changes in chromatin organization represent the initial steps of tumorigenesis: a transmission electron microscopy study, Lusik Cherkezyan1+, Yolanda Stypula-Cyrus1+, Hariharan Subramanian1, Craig White1, Mart Dela Cruz2, Ramesh K Wali2, Michael J Goldberg3, Laura K Bianchi3, Hemant K Roy2 and Vadim Backman1*, BMC Cancer, Cell and molecular biology, Volume 14, 2014,* Corresponding author: Vadim Backman, + Equal contributors, 1 Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, USA , 2 Department of Medicine, Boston Medical Center, Boston, Massachusetts 02118, USA ,3 Department of Internal Medicine, NorthShore University HealthSystem, Evanston, Illinois 60201, USA
  • Visualizing  compaction of polysomes in bacteria, Nicolas Cougot1, Anne-Elisabeth Molza1, Jeremy Delesques1, Emmanuel Giudice1, Annie Cavalier1, Jean-Paul Rolland1, Gwennola Ermel2, Carlos Blanco2, Daniel Thomas1 and Reynald Gillet1,3*, ELSEVIER Journal of Molecular Biology, Volume 426, Issue 2, 2013, 1 Team Translation and Folding, Université de Rennes 1, UMR CNRS 6290 IGDR, Campus de Beaulieu, 35042 Rennes Cedex, France, 2 Université de Rennes 1, EA 1254, Campus de Beaulieu, 35042 Rennes Cedex, France, 3 Institut Universitaire de France
  • Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy, C.C. Chien a,b,, P.Y. Tsenga, H.H. Chena, T.E. Huaa, S.T. Chena, W.H. Lenga, C.H. Wanga, Y.Hwua,b,c,d,*, G.C. Yine, K.S. Liangf, F.R. Chenb, Y.S. Chug,H.I. Yehh, Y.C. Yangh, C.S. Yangh, G.L. Zhangj, J.H. Jek, G. Margaritondol, ELSEVIER, Biotechnology Advances, Volume 32, Issue 3, May-June 2013, a Institute of Physics, Academia Sinica, Taipei 115, Taiwan, b Engineering and System Science, National Tsing Hua University, Hsinchu 300, Taiwan, c Institute of Optoelectronic Sciences, National Taiwan Ocean University, Keelung 202, Taiwan, d Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan 701, Taiwan, e National Synchrotron Radiation Research Center, Hsinchu 300, Taiwan, f Electrophysics Department, National Chiao Tung University, Hsinchu 300, Taiwan, g NSLS-II, Brookhaven National Laboratory, Upton, NY 11973‐5000, USA, h Mackay Memorial Hospital, Taipei 104, Taiwan, i Center for Nanomedicine, National Health Research Institutes, Miaoli 350, Taiwan, j Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China, k X-ray Imaging Center, Pohang University of Science and Technology, Pohang 790‐784, Republic of Korea, l Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerlandhttp://www.sciencedirect.com/science/article/pii/S073497501200081X
  • The transmembrane protein Macroglobulin complement-related is essential for septate junction formation and epithelial barrier function in Drosophila, Tilmann Bätz++, Dominique Förster*,++ and Stefan Luschnig§ Institute of Molecular Life Sciences and PhD Program in Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland, Development,141, received August 5, 2013, accepted December 8, 2013, *Present address: Max Planck Institute of Neurobiology, Am Klopferspitz 18, D-82152 Martinsried, Germany, ++These authors contributed equally to this work, §Author for correspondence
  • Correlative Light- and Electron Microscopy with chemical tags, Mario Perkovic a, Michael Kunz a , Ulrike Endesfelder b, Stefanie Bunse c, Christoph Wigge a, Thou Yu a, Victor-Valentin Hodirnau a, Margot P.Scheffer a. Anja Sybert a, Sebastian Malkusch b, Erin M. Schuman c, Mike Heilemann b, Achilleas S. Frangakis a,*, Journal of Structural Biology, Volume 186, Issue 2, May 2014, aGoethe University Frankfurt, Buchmann Institute for Molecular Life Sciences and Institute for Biophysics, Max-von-Laue Str. 15, 60438 Frankfurt am Main, Germany, bGoethe University Frankfurt, Institute for Physical and Theoretical Chemistry, Max-von-Laue Str. 13, 60438 Frankfurt am Main, Germany, cMax-Planck-Institute for Brain Research, Max-von-Laue Str. 4, 60438 Frankfurt am Main, Germany

  • Deep Sequencing of Myxilla (Ectyomyxilla) methanophila, an Epibiotic Sponge on Cold-Seep Tubeworms, Reveals Methylotrophic, Thiotrophic, and Putative Hydrocarbon-Degrading Microbial Associations , Shawn M. Arellano & On On Lee & Feras F. Lafi & Jiangke Yang & Yong Wang & Craig M. Young & Pei-Yuan Qian, SpringerLink, Microbiial Ecology, February 2013, Volume 65, Issue 2, pp450-461, http://link.springer.com/article/10.1007/s00248-012-0130-y#
  • Role of CEACAM1 and CEACAM20 in an In Vitro Model of Prostate Morphogenesis,Hui Zhang - City of Hope Irell & Manella Graduate School of Biological Sciences, Duarte, California, United States of America, Hui Zhang, John E. Shively - Department of Immunology, Beckman Research Institute of City of Hope, Duarte, California, United States of America, Andreas Eisenried - Anästhesiologische Klinik, Universitätsklinikum Erlangen, Erlangen, Germany, Wolfgang Zimmermann - Labor fürTumorimmunologie, LIFE-Zentrum, München, Germany, PLOS One, January 24, 2013, http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0053359 
  • Immunohistochemical localization of caffeine in young Camellia sinensis (L.) O. Kuntze (tea) leaves, Shane V. van Breda1,2, Chris F. van der Merwe3, Hannes Robbertse4, Zeno Apostolides2, 2Department of Biochemistry, 3Laboratory for Microscopy and Microanalysis,4Department of Plant Production and Soil Science, University of Pretoria, Lynnwood Road,Hatfield, Pretoria, 0002, Gauteng, South Africa. Spinger Link, Planta, March 2013, Volume 237, Issue 3, pp 849-858 http://link.springer.com/article/10.1007/s00425-012-1804-x#
  • Changes of ultrastructure and stress tolerance of Vibrio parahaemolyticus upon entering viable but nonculturable state, Chao-Ping Su, Wann-Neng Jane, Hin-chung Wong, Department of Microbiology, Soochow University, Taipei, Taiwan, Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China, International Journal of Food Microbiology, Volume 160, Issue3, 2013, Pages 360 - 366,
  • Pigment production by a new thermotolerant microalga Coelastrella sp.F50, Che-Wei Hu, Lu-Te Chuang, Po-Chien Yu, Ching-Nen Nathan Chen, Institute of Marine Biology, National Sun Yat-sen University, Kaohsiung, 804, Department of Biotechnology, Yuanpei University, Hsinchu, Asia Pacific Ocean Study Center, National Sun Yat-sen University, Kaohsiung, Taiwan, Elsevier, Food Chemistry, 2012
  • Quantitative evaluation of freeze-substitution effects on preservation of nuclear antigens during preparation of biological samples for immunoelectron microscopy, Margarita A. Sobol, Vlada V. Philimonenko, Anatoly A. Philimonenko, Pavel Hozák, Springer Link, Histochemistry and Cell Biology, July 2012, Volume 138, Issue 1, pp 167 - 177 http://link.springer.com/article/10.1007%2Fs00418-012-0931-6?LI=true#