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Leica DMS1000 Quality Control

Low-to-Mid Range Magnification Digital Microscope Leica DMS1000

DMS1000

The Leica DMS1000 offers telecentric optics. What exactly does this mean?

Put your thumb in front of your eye and look at a tree, car or house. They appear to be about the same size, while in reality, we know your thumb is much smaller. This disproportionate perception occurs because our eyes are not telecentric, so objects closer to our eyes appear larger. Non-telecentric metrology solutions inaccurately measure objects closer to the camera as larger than objects farther away from the camera. Because the Leica DMS1000 offers fully-telecentric optics, users can rely on the system for accurate measurements. Please keep in mind that the Leica DMS1000 only is telecentric with an adapter and the proper objectives as mentioned in the user manual.

What is the difference between parcentric and telecentric?

Parcentric optics help keep the area of interest centered though-out the entire zoom range. This saves time as users need not move the sample for each zoom setting. Telecentric optics uses rays for imaging that leave the object plane at a perpendicular angle. This helps to avoid lateral shifts of the field of view and/or variations of the imaging scale during focusing as the perspective is perpendicular over the whole object field.

What is the benefit of being parfocal and parcentric and how do I set this up?

A parfocal and telecentric system enables users to change the magnification without having to refocus or reposition the sample on every zoom setting. The setup is straightforward and simple. First center your sample on the monitor at the lowest zoom setting and the bring it into focus. Then move the magnification to the highest setting and again the bring the image back into focus. The specimen or area of interest will remain centered and in focus throughout the zoom range.

Since the Leica DMS1000 does not utilize eyepieces, how is magnification calculated?

With the Leica DMS1000, a monitor takes the place of eyepieces. As a rule of thumb, the larger the monitor, the greater the magnification factor. The magnification range of the Leica DMS1000 system provides ranges from about 3x to 300x using a 10” and 22” monitor respectively. LAS offers the ability to display the total digital microscope system magnification based on monitor size, and most importantly, an accurate scale bar.

I need to work in a sterile environment. How can I clean, disinfect or sterilize the Leica DMS1000 for this?

We recommend using a damp cloth, with water and alcohol (max. 70%) solution, on external surfaces and the glass objectives.

The Leica DMS1000 offers FlexAperture technology. What exactly this do for me?

FlexAperture automatically maintains a homogenous illumination throughout the zoom range of the Leica DMS1000. This keeps the camera exposure time constant, thereby saving time and increasing efficiency.

Can I run the Leica DMS1000 on a Mac OS system?

Yes, the system can work on a Mac OS using the Leica Acquire (version 2.0) software. With Leica Acquire you can easily capture images. If you wish to use Leica Applications Suite on a Mac, you will need to install Apple BootCamp and a compatible Windows OS to run in PC mode.

Can I use Leica Application Suite (LAS) with the Leica DMS1000?

Yes, users can run the Leica DMS1000 with LAS and many of its optional modules. LAS is included in the Leica DMS1000 package.

Which LAS modules are compatible with the Leica DMS1000?

All LAS modules except Leica LAS Power Mosaic are technically compatible.

Can I acquire extended focus / panoramic images with the Leica DMS1000?

Absolutely. Leica LAS Live Image Builder will produce extraordinary panoramic and extended focus images when the Leica DMS1000 is configured with a manual XYZ stage and focus. Leica LAS MultiStep can automatically acquire outstanding panoramic images in conjunction with a motorized stage. Stunning 3D qualitative and quantitative images can also be acquired when configured with a Leica Motor Focus and Leica LAS Montage.

Can I create my own overlays for the Leica DMS1000 microscopes?

Yes you can, up to ten custom overlays can be imported to the Leica DMS1000. Step by step instructions and templates are available on the Leica DMS1000 product website under Downloads / Software.

How is the Leica DMS1000 different from the Leica DMS1000 B?

The Leica DMS1000 B is a complete outfit that includes the Leica DMS1000 but has been certified for in-vitro-applications like in-vitro-fertilization (IVF).

Can I update the firmware on my Leica DMS1000?

Yes. From time to time new firmware updates will be made available to improve performance and/or add new features. This can be easily done without the need to return the unit. New firmware updates will be posted on the Leica DMS1000 product website under Downloads / Firmware. Step by step instructions are also provided with the download.

What is the benefit of telecentric optics?

Because the Leica DMS1000 offers fully-telecentric optics, users can rely on the system for accurate measurements. Please keep in mind that the Leica DMS1000 only is telecentric with an adapter and the proper objectives as mentioned in the user manual.

What makes a digital microscope a digital microscope?

A digital microscope is a microscope without eyepieces. Instead of eyepieces, it has a digital camera that acquires live images which are visible on a monitor.

What are digital microscopes suitable for?

Digital microscopes are suitable for a wide range of technical applications. They are ideal instruments for fast and easy documentation of parts and samples during manufacturing, as well as assembly, quality control, and failure analysis of manufactured parts.

What makes digital microscopes ideal instruments for these tasks?

Digital microscopes are designed for rapid acquisition of high quality images in quality control, failure analysis, and documentation. These images must be calibrated, which means that measurement data taken from the image, such as dimensions, are correctly quantified and accurate. Suitable software makes sure that images can be analyzed easily. For example measurements can be made with the image, it can then be annotated and stored in a standard format for later use in reports, presentations, publications, or discussion with colleagues or customers.

What makes image data of digital microscopes from Leica Microsystems correct and reliable?

The precise calibration data, such as dimensions in the x, y, and z directions, and crucial parameters, such as magnification and the corresponding scale bar, are recorded directly with each image. These features ensure that image analysis leads to accurate measurement and quantification data.