Rolf T. Borlinghaus, Dr.
Rolf Borlinghaus was born 1956 in Grötzingen, Germany. After his diploma in Biology he worked on electrogenic steps of the Na/K-ATPase by laser-induced release of ATP from a caged compound at Peter Läuger’s Laboratory in the Biophysics Department, University Konstanz, Germany from where he was promoted to Dr.rer.nat. in 1988. He started working as a Product Manager for research Fluorescence and confocal Microscopes with Carl Zeiss, Oberkochen in 1990 and continued to tackle this challenge at Leica in 1997 (at that time Leica Lasertechnik, Heidelberg). For personal insights, in 2007, Rolf Borlinghaus dispensed his managerial responsibilities and is now supporting the confocal marketing group as scientific advisor in a half-time position. The other half is dedicated to relations, food, music, books and botany.
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Tutorial
19. Apr 2012 Institute: Leica Microsystems, Wetzlar, GermanySeeing is believing – and measuring is knowing. Microscopes generate images that are not only used for illustration, but are also subject to quantification. More advanced techniques use illumination patterns (without image formation) or do not generate an image at all – but are still microscopical… Read article
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Article
10. Feb 2012 Institute: Leica Microsystems, Wetzlar, GermanyOptogenetics is a technique that allows light-controlled responses of transfected cells. The cells are genetically modified by introduction of genes that code for light-induced channels or ion pumps. The term optogenetics denotes the light control feature introduced by genetic engineering. Read article
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Tutorial
09. Dec 2011 Institute: Leica Microsystems, Wetzlar, GermanyFluorescence Microscopy usually employs incident light illumination. This requires a device that directs the light for illumination into the sample and transmits the light emitted by the sample to the detection system. In the past, various types of mirrors were the only option. Today, the acousto… Read article
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Tutorial
09. Dec 2011 Institute: Leica Microsystems, Wetzlar, GermanyTo separate fractions of the emission for recording channels that reproduce the emission of individual fluorochromes, it is necessary to spatially disperse the emission spectrally. This is possible by employing dichroic mirrors or a genuine dispersive element like a prism or a grating. Read article
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Tutorial
09. Dec 2011 Institute: Leica Microsystems, Wetzlar, GermanyFluorescence excitation needs specifically colored light. In confocal microscopy, multiline lasers or laser batteries are classically used. This requires devices that pick the requested lines fitting the currently employed fluorochromes. Intensity control is a second task that must be accomplished. Read article
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Article
09. Nov 2011 Institute: Leica Microsystems, Wetzlar, GermanyNeurobiology, the science of nerves and the brain, has mainly been driven forward in the last 200 years by microscopic investigations. The structures of cellular and subcellular structures, interaction and the three-dimensional assembly of neurons were made visible by various microscopy techniques.… Read article
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Tutorial
03. Aug 2011 Institute: Leica Microsystems, Wetzlar, GermanyFast True Confocal Scanning reduces photobleaching and increases the fluorescence yield at identical acquisition times. The long-lasting triplet state (or any other “dark state”) is less populated when the illumination is applied in shorter pulses at the same intensity. Consequently, more… Read article
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Tutorial
01. Jul 2011 Institute: Leica Microsystems, Wetzlar, GermanySTED uses a differential method of two different diffraction patterns, where one pattern excites and the second pattern de-excites fluorochromes. The residual excited area is controllable by intensity down to (theoretically) zero – unlimited resolution. Read article
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Tutorial
22. Jun 2011 Institute: Leica Microsystems, Wetzlar, GermanyTrue confocal scanning microscopes (TCS) use a variable detection pinhole. Good optical sectioning tries to use just the inner core of the PSF. Read article
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Tutorial
21. Jun 2011 Institute: Leica Microsystems, Wetzlar, GermanyConfocal microscopes are employed to optically slice comparably thick samples. Read article
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Tutorial
21. Jun 2011 Institute: Leica Microsystems, Wetzlar, GermanyTrue confocal scanning microscopy (TCS) requires bright diffraction-limited illumination. Read article
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Tutorial
09. May 2011 Institute: Leica Microsystems, Wetzlar, Germany“Confocal Microscopy” refers to a particular optical microscope that allows recording optical sections. Optical sectioning is achieved in a confocal system by illuminating and observing a single diffraction limited spot. Read article