Marko Lampe, Dr.

Marko Lampe joined Leica Microsystem in Wetzlar, Germany, in March 2011 as Application Manager for widefield super-resolution microscopy and cell biology applications. He studied Biochemistry at the University of Hanover and focused on single molecule detection fluorescently labeled motor-proteins with TIR-FM in vitro.

During his Ph.D. thesis he studied the entry of fluorescently labeled HIV-1 particles into the host cell at the University Hospital of Heidelberg, Germany. From 2008 until 2011, he worked as a postdoctoral fellow at the MRC Laboratory of Molecular Biology (LMB) in Cambridge, UK, studying clathrin-mediated endocytosis of GPCRs with live cell fluorescence microscopy.

Marko.Lampe@leica-microsystems.com

Sample Preparation for GSDIM

The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as 20 nanometers. GSDIM is suitable for a wide range of samples.

Super-Resolution

Jul 27, 2012

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Widefield Super-Resolution with GSDIM

Great advancements in biology have been possible by using fluorescence microscopy. So far, the resolution of the images was limited due to physical constraints. In the past couple of years, new methods evolved circumventing these limitations and bringing fluorescence microscopy to a new level of resolution, boosting the possibilities in science with fluorescence microscopes.

Super-Resolution

Jul 12, 2012

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Video Tutorial: How to Optimize Sample Preparation for GSD Microscopy

This video tutorial presents a reliable way to produce samples for super-resolution GSD imaging with a special focus on the mounting step. Stable and flat mounting of the coverslip increases the performance of the overall system, leading to an improved resolution of the GSD image.

Super-Resolution

Jan 13, 2012

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Webinar: Applications, Labeling Strategies and Fluorophores for Super-Resolution

The free online webinar on super-resolution presented by Leica Microsystems in association with Microscopy & Analysis took place on Tuesday, 15 November 2011. Register and view the webinar on demand.

Nov 15, 2011

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How Widefield Super-Resolution GSDIM Images are Created

The localization microscopy technique GSDIM is a proven technology to achieve super-resolution images with a resolution of up to 20 nm. In the following tutorial we will describe the basic principles and features of GSDIM.

Super-Resolution

Oct 31, 2011

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Step by Step Guide to the Molecular Basics of GSDIM Microscopy

Ground state depletion microscopy followed by individual molecule return (GSDIM) is a super-resolution technique based on single molecule localization (Localization Microscopy). To localize single molecules and create a high resolution image the ensemble of overlapping fluorophores (in a diffraction-limited setup) has to be broken up. Individual fluorophores must be temporally "separated" to allow high precision detection of single molecules. This tutorial will explain the molecular basics of GSDIM.

Super-Resolution

Oct 28, 2011

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Koehler Illumination

This tutorial demonstrates the basic steps for setting up a correct illumination of specimen with transmitted (and reflected) light.

Basics in Microscopy

May 31, 2011

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