Beam Paths of a Continuous Wave STED Microscope

May 14, 2012

This tutorial gives on overview of the excitation, depletion and detection path in a continuous wave (CW) STED microscope.

Instead of synchronized pulsed excitation and STED pulses, CW STED uses a continuous-wave light source (or a non-synchronized pulsed source) for excitation of the fluorochromes. TCS STED CW applies a continuous wave laser at 592 nm to de-excite the fluorophores and, therefore, works with green dyes and yellow fluorescent proteins such as Alexa 488, Oregon Green 488 and eYFP.

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