Clarifying Tissue Clearing

January 26, 2017

Biological specimens are intrinsically three dimensional; however because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to eliminate the scatter by “clearing” the tissue have been ongoing for over a century, there have been a large number of recent innovations. This review introduces the physical basis for light-scatter in tissue, describes the mechanisms underlying various clearing techniques, and discusses several of the major advances in light microscopy for imaging cleared tissue.

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Richardson DS, Lichtman JW:
Clarifying Tissue Clearing

Cell 162(2): 246–257 (2015). DOI:10.1016/j.cell.2015.06.067

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