Hand creams having different water contents were applied into the 100 μm cavities of two 3 mm type A sample carriers which were then closed cream sides inwards. The sample assembly was high pressure frozen with a Leica EM HPM100 and moved to a cooled Leica EM VCT100 loading station. The closed carriers were mounted on a universal sample carrier, withdrawn into an EM VCT100 shuttle then transferred into a Leica EM BAF060.
Samples were fractured by pushing the top carrier off with the fracturing knife at -140°C, then they were high angle rotary shadowed with platinum/carbon. The samples were again withdrawn into the EM VCT100 shuttle and then moved to the cryo-SEM. They were imaged with a Hitachi S4700 FE-SEM with the EM VCT100 CryoStage at –125°C.