CRISPR has become one of the biologist’s favorite ways for deleting, replacing, or editing DNA, and much of the conversation about CRISPR-Cas9 has revolved around its potential for gene editing in health and disease. This webinar will showcase how CRISPR has also begun to revolutionize our understanding of how genomes work and will discuss the potential of CRISPR imaging tools to study genetic elements within living cells. Two leaders in this field, Gene Yeo from UCSD and Bo Huang from UCSF, discuss techniques, technology, and insights on CRISPR imaging.
In this webinar, we show that optical clearing drastically increases the signal-to-noise ratio and staining quality, thus enabling STED nanoscopy of the subtlest elements of the kidney. In this way we show that optical clearing is not only a sample preparation technique to consider when imaging large mm-scale samples, but could also be fruitful when imaging at the nanoscale. Furthermore, the increased transparency of the optically cleared sample enables volumetric 3D STED imaging at sub-diffraction-limited resolution.
The webinar will provide an overview of the latest advances in Cryo CLEM, which acts as a powerful interface by combining the best of the light and electron microscopy worlds to overcome their independent barriers and determine the location of fluorescent labelled structures within the landscape of an electron micrograph and showcase how Cryo CLEM adds additional value to quantitative 3D imaging and tomography.
Cancer can affect various organs and is caused by mutations of the DNA. A prerequisite, to explore and understand underlying gene-mutations involved in the development of a definite type of cancer, is the extraction of pure sample material, which is challenging. In this webinar, we will present how to extract 100% pure cancer tissue for DNA analysis with laser microdissection (LMD). Using tissue samples from human kidney cancer patients as an example, this webinar will provide an overview of the practical considerations when preparing a workflow to obtain highly pure material with the LMD microscope for further molecular analysis.
In this seminar we will provide an overview about the latest advances in fluorescence microscopy. You will learn how you can use widefield and confocal microscopes to help you understand life’s questions down to tiny details, at high speed and state-of-the-art image quality both in living and fixed samples.
Participants will receive an overview on the concept of fluorescence-guided surgery (FGS) for the resection of brain and spinal cord tumors. Neurosurgeons will understand the current technologies available for FGS and the different tumor types that can undergo FGS. Safety, diagnostic accuracy, and Level 1 evidence in support of FGS will also be discussed.
In this webinar, we will introduce the features and applications of the Leica EM ICE high pressure freezer with light stimulation, emphasizing the physics and technical solutions behind the user interface. We will discuss parameters such as dwell time, pressure and temperature correlation, how they are measured and what the displayed data means. We will also explain how light stimulation is synchronized with the freezing process to give you millisecond precision in your experiments.
In this webinar you will learn about the advantages of using laser mikcrodissection for precise, contamination-free isolation of specific cell types in various applications. Using pancreatic cancer tissue sections from patients and ortothopic xenografts as examples, this webinar will provide an overview of the scientific and practical considerations for obtaining highly pure material for further molecular analysis in the field of pharmacological studies to overcome key mechanisms of resistance in pancreatic cancer.
Digital microscopy ensures fast and efficient quality control. In this presentation, attendees will learn how to use digital microscopes for the accurate analysis of complex components, and for the rapid generation of 2D and 3D documentation.
During this webcast, we will present recent advances in targeted cell labelling, tissue clearing, and fluorescence imaging methods for the study of brain function. These exciting methods are helping to accelerate the understanding of how individual cells and complex neural circuits interact both structurally and functionally.