Cancer can affect various organs and is caused by mutations of the DNA. A prerequisite, to explore and understand underlying gene-mutations involved in the development of a definite type of cancer, is the extraction of pure sample material, which is challenging. In this webinar, we will present how to extract 100% pure cancer tissue for DNA analysis with laser microdissection (LMD). Using tissue samples from human kidney cancer patients as an example, this webinar will provide an overview of the practical considerations when preparing a workflow to obtain highly pure material with the LMD microscope for further molecular analysis.
In this seminar we will provide an overview about the latest advances in fluorescence microscopy. You will learn how you can use widefield and confocal microscopes to help you understand life’s questions down to tiny details, at high speed and state-of-the-art image quality both in living and fixed samples.
Participants will receive an overview on the concept of fluorescence-guided surgery (FGS) for the resection of brain and spinal cord tumors. Neurosurgeons will understand the current technologies available for FGS and the different tumor types that can undergo FGS. Safety, diagnostic accuracy, and Level 1 evidence in support of FGS will also be discussed.
In this webinar, we will introduce the features and applications of the Leica EM ICE high pressure freezer with light stimulation, emphasizing the physics and technical solutions behind the user interface. We will discuss parameters such as dwell time, pressure and temperature correlation, how they are measured and what the displayed data means. We will also explain how light stimulation is synchronized with the freezing process to give you millisecond precision in your experiments.
In this webinar you will learn about the advantages of using laser mikcrodissection for precise, contamination-free isolation of specific cell types in various applications. Using pancreatic cancer tissue sections from patients and ortothopic xenografts as examples, this webinar will provide an overview of the scientific and practical considerations for obtaining highly pure material for further molecular analysis in the field of pharmacological studies to overcome key mechanisms of resistance in pancreatic cancer.
During this webcast, we will present recent advances in targeted cell labelling, tissue clearing, and fluorescence imaging methods for the study of brain function. These exciting methods are helping to accelerate the understanding of how individual cells and complex neural circuits interact both structurally and functionally.
In this webinar the sample preparation workflow including the Ultramicrotome Leica EM UC7, its cryo-chamber Leica EM FC7 and the pre-preparation system Leica EM TXP will be given. The main part of this webinar will cover tips and tricks to reveal the internal structure of composites and materials being investigated with TEM and STEM. Differences between Focused Ion Beam (FIB) and Ultramicrotomed samples will be shown and explained.
This webinar presented by Dr Marco Fritzsche, University of Oxford, and Jennifer Horner, PhD, Leica Microsystems, you will learn about how to use Fluorescence Recovery After Photo-bleaching (FRAP) microscopy to study protein dynamics.
In this webinar, presented by Dr. Imre Gaspar of the Developmental Biology Unit at EMBL, you will learn: importance of mRNA localization and function of mRNPs, advantages of using fluorogenic FIT probes to visualize mRNPs in vivo and in fixed specimen, and how super-resolution microscopy can identify factors required for mRNP biogenesis.