The modern microscope has evolved into the instrument of choice for extracting fast and reliable measurement data in the R&D, quality control, and production workplaces. However, many instruments are not fully optimized to produce accurate, repeatable, and reproducible measurement data.
The discovery of green fluorescent protein a half century ago heralded a new and explosive era in microscopy, forever changing the landscape for biology imaging. The ability to fuse a genetically encoded fluorescent probe to an almost-unlimited variety of proteins has enabled scientists to investigate signaling pathways and the movement of intracellular proteins in living cells with unprecedented detail, particularly when coupled with powerful widefield fluorescence and confocal microscopy techniques.
This webinar will illustrate results obtained by biochemical, Epifluorescence, TIRFM, Confocal and GSD techniques. Depending on the aim of experimental question, different imaging techniques deliver insights into varying aspects of intracellular pathways. To achieve "True-to-detail imaging" of the spatial arrangement of proteins and other biomolecules in cells, GSDIM achieves resolutions up to 20 nm in x and y direction – beyond the diffraction limit of light microscopy. But Super-resolution microscopy can be applied in the axial (z-) direction, too. A recent commercial implementation of the astigmatism approach will be discussed in more detail during this webinar.
Brain research using confocal and multiphoton microscopy.
A deeper understanding of brain function requires visualization of the complex architecture of neurons and their connections. In this special webinar, two researchers working at the cutting-edge of brain imaging will discuss their applications of the latest confocal and two-photon microscopy techniques. From fluorescent labeling and imaging of neural circuits using the Brainbow system to in vivo imaging of brain structure, function, and blood flow, this webinar will give researchers a deeper appreciation of the potential for new microscopy methods to unlock the secrets of the cellular world. Unique insights into sample handling and processing of multicolor images will also be presented, and attendees will have the opportunity to ask questions.
The beauty of cells, magnified and resolved by light microscopy, has been fascinating investigators since the 19th century. Today, functional research in living cells is often a prerequisite for biological studies. And keeping the cells close to the necessary conditions whilst under microscopical observation is key. In this webinar, Dr. Christoph Bauer will share his experiences of live cell imaging and talk about how the microscopy challenges, such as optical aberrations at 37°C or focus drift in long-term experiments, can be addressed.
In recent years, new methods and techniques aimed at deciphering the inner workings of cells have provided unique insights, and changed the way we think about, as well as approach, modern cell biology research. With these advances in mind, the 2012 BioTechniques Virtual Symposium will focus directly on the latest approaches being used to characterize cell function and phenotype.
Doing more with fewer resources is today’s standard, and the metallurgical/quality-control laboratory is no exception. Digitizing the metallurgical laboratory provides software tools that increase overall efficiency and accuracy of microstructure evaluation in accordance with ASTM and ISO standards, automate the generation of quality reports, and provide a structured interface for archiving images and related data. This webinar will provide a comprehensive overview of how these digital tools can help optimize workflow and save time and money.
Live cell imaging is one tool in the cell biologist's tool box. There are, however, a variety of technologies and methods to achieve this. In this webinar, three practicing researchers discuss: Their use of live cell imaging and how it has helped them achieve publishable results.
Leica Microsystems recently introduced their next generation of confocal, the Leica TCS SP8 – a truly configurable platform that allows you to create your confocal exactly as you need it. As your scientific research develops further, so you need a system that evolves, adapts and reconfigures. What you discover today will be the foundation of your discoveries tomorrow.
This webinar is for any biologist interested in learning about how cells balance resources and make the decision between redirecting resources and initiating death. It will be a great opportunity to learn how the experts are studying these processes and how you can monitor and image each process.