Laser microdissection plays a key role in these examinations. Besides electrophysiological measurements of signals in single neurons, the contents of the neurons can be sucked, enabling molecular biological analysis to be acquired as well as electrophysiological data. However, this process is very time-consuming and restricted to laboratory animals.
Using laser microdissection it is possible to collect a large number of single cells, analyze their molecular composition and compare them with cells whose contents have been sucked after electrophysiological measurements. This means that cells “of the same content” are indirectly assignable to the same signals. This step can also be extended further to human post-mortem single cells that can only be collected specifically from certain sub-regions by laser microdissection. In this paper, murine and human dopaminergic neurons were used for analysis, allowing conclusions to be drawn on human pathomechanisms.